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N-WASP antibody

Anti-N-WASP antibody used in Western Blot (WB). GTX32762
Anti-N-WASP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32762
Anti-N-WASP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32762
Anti-N-WASP antibody used in IHC (Paraffin sections) (IHC-P). GTX32762
Anti-N-WASP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32762
Anti-N-WASP antibody used in IHC (Paraffin sections) (IHC-P). GTX32762
Anti-N-WASP antibody used in IHC (Paraffin sections) (IHC-P). GTX32762

Cat. No. GTX32762

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all N-WASP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:100
IHC-P 1:50 - 1:100
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human WASL (NP_003932.3).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

WASP like actin nucleation promoting factor , N-WASP , NWASP , WASPB

Cellular Localization

Cytoplasm, cytoskeleton,Nucleus,Cytoplasm

Background

This gene encodes a member of the Wiskott-Aldrich syndrome (WAS) protein family. Wiskott-Aldrich syndrome proteins share similar domain structure, and associate with a variety of signaling molecules to alter the actin cytoskeleton. The encoded protein is highly expressed in neural tissues, and interacts with several proteins involved in cytoskeletal organization, including cell division control protein 42 (CDC42) and the actin-related protein-2/3 (ARP2/3) complex. The encoded protein may be involved in the formation of long actin microspikes, and in neurite extension. [provided by RefSeq, Jul 2013]

Database

Research Area

DATA IMAGES

Anti-N-WASP antibody used in Western Blot (WB). GTX32762

GTX32762 WB Image

WB analysis of various sample lysates using GTX32762 N-WASP antibody.
Dilution : 1:3000
Loading : 25μg per lane

Anti-N-WASP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32762

GTX32762 ICC/IF Image

ICC/IF analysis of L929 cells using GTX32762 N-WASP antibody.
Blue : DAPI
Dilution : 1:100

Anti-N-WASP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32762

GTX32762 ICC/IF Image

ICC/IF analysis of C6 cells using GTX32762 N-WASP antibody.
Blue : DAPI
Dilution : 1:100

Anti-N-WASP antibody used in IHC (Paraffin sections) (IHC-P). GTX32762

GTX32762 IHC-P Image

IHC-P analysis of mouse heart tissue using GTX32762 N-WASP antibody.
Dilution : 1:100

Anti-N-WASP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32762

GTX32762 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX32762 N-WASP antibody.
Blue : DAPI
Dilution : 1:100

Anti-N-WASP antibody used in IHC (Paraffin sections) (IHC-P). GTX32762

GTX32762 IHC-P Image

IHC-P analysis of rat brain tissue using GTX32762 N-WASP antibody.
Dilution : 1:100

Anti-N-WASP antibody used in IHC (Paraffin sections) (IHC-P). GTX32762

GTX32762 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX32762 N-WASP antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399