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N-myc interactor antibody [N1C3]

Anti-N-myc interactor antibody [N1C3] used in Western Blot (WB). GTX102866
Anti-N-myc interactor antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX102866

Cat. No. GTX102866

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all N-myc interactor products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Positive Control

293T , Raji

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.69 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human N-myc interactor. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951008

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

N-myc and STAT interactor

Cellular Localization

Cytoplasm

Background

NMYC interactor (NMI) encodes a protein that interacts with NMYC and CMYC (two members of the oncogene Myc family), and other transcription factors containing a Zip, HLH, or HLH-Zip motif. The NMI protein also interacts with all STATs except STAT2 and augments STAT-mediated transcription in response to cytokines IL2 and IFN-gamma. The NMI mRNA has low expression levels in all human fetal and adult tissues tested except brain and has high expression in cancer cell line-myeloid leukemias. [provided by RefSeq]

Database

DATA IMAGES

Anti-N-myc interactor antibody [N1C3] used in Western Blot (WB). GTX102866

GTX102866 WB Image

Sample(30 μg of whole cell lysate)
A:293T
B:Raji(GTX27908)
12% SDS PAGE
GTX102866 diluted at 1:500

Anti-N-myc interactor antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX102866

GTX102866 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human endo mitral ovarian cancer, using NMI(GTX102866) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169