GeneTex
United States (US)

N-myc interactor antibody [N1C3]

GTX102866_IHC_19011506_923.jpg
GTX102866_WB_19011506_375.jpg

Cat No. GTX102866

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Raji

Predict Reactivity

Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.69 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human N-myc interactor. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

N-Myc And Stat Interactor,Nmi

Cellular Localization

Cytoplasm

Background

NMYC interactor (NMI) encodes a protein that interacts with NMYC and CMYC (two members of the oncogene Myc family), and other transcription factors containing a Zip, HLH, or HLH-Zip motif. The NMI protein also interacts with all STATs except STAT2 and augments STAT-mediated transcription in response to cytokines IL2 and IFN-gamma. The NMI mRNA has low expression levels in all human fetal and adult tissues tested except brain and has high expression in cancer cell line-myeloid leukemias. [provided by RefSeq]

Database

DATA IMAGES
GTX102866_IHC_19011506_923.jpg

GTX102866 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human endo mitral ovarian cancer, using NMI(GTX102866) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102866_WB_19011506_375.jpg

GTX102866 WB Image

Sample(30 μg of whole cell lysate)
A:293T
B:Raji(GTX27908)
12% SDS PAGE
GTX102866 diluted at 1:500

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169