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NAB2 antibody

Anti-NAB2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103581
Anti-NAB2 antibody used in Immunoprecipitation (IP). GTX103581
Anti-NAB2 antibody used in IHC (Paraffin sections) (IHC-P). GTX103581
Anti-NAB2 antibody used in Western Blot (WB). GTX103581

Cat No. GTX103581

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

A431

Predict Reactivity

Rat, Bovine, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NAB2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

NGFI-A binding protein 2 , MADER

Cellular Localization

Nucleus

Background

This gene encodes a member of the family of NGFI-A binding (NAB) proteins, which function in the nucleus to repress transcription induced by some members of the EGR (early growth response) family of transactivators. NAB proteins can homo- or hetero-multimerize with other EGR or NAB proteins through a conserved N-terminal domain, and repress transcription through two partially redundant C-terminal domains. Transcriptional repression by the encoded protein is mediated in part by interactions with the nucleosome remodeling and deactylase (NuRD) complex. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-NAB2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103581

GTX103581 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NAB2(GTX103581) antibody at 1:500 dilution.

Anti-NAB2 antibody used in Immunoprecipitation (IP). GTX103581

GTX103581 IP Image

Immunoprecipitation of NAB2 protein from Jurkat whole cell extracts using 5 μg of NAB2 antibody (GTX103581).
Western blot analysis was performed using NAB2 antibody (GTX103581).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-NAB2 antibody used in IHC (Paraffin sections) (IHC-P). GTX103581

GTX103581 IHC-P Image

NAB2 antibody detects NAB2 protein at nucleus on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
NAB2 antibody (GTX103581) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NAB2 antibody used in Western Blot (WB). GTX103581

GTX103581 WB Image

Sample (30 ug of whole cell lysate)
A: A431
7.5% SDS PAGE
GTX103581 diluted at 1:2000

REFERENCE
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REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169