GeneTex
United States (US)

NADE antibody [N1C3]

GTX102376_WB_19011506_866.jpg
GTX102376_40128_IFA_19011506_953.jpg
GTX102376_IHC_19011506_858.jpg

Cat No. GTX102376

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

13 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji

Predict Reactivity

Mouse, Bovine, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.38 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NADE. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Brain Expressed X-Linked 3,Bex,Dxs6984E,Hgr74,Nade,Ngfrap1,Bex3

Cellular Localization

Nucleus , Cytoplasm

Background

May be a signaling adapter molecule involved in p75NTR-mediated apoptosis induced by NGF. Plays a role in zinc-triggered neuronal death (By similarity). May play an important role in the pathogenesis of neurogenetic diseases.

Database

Research Area

DATA IMAGES
GTX102376_WB_19011506_866.jpg

GTX102376 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: A431 (GTX27909)
C: H1299
15% SDS PAGE
GTX102376 diluted at 1:1000

GTX102376_40128_IFA_19011506_953.jpg

GTX102376 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NADE(GTX102376) antibody at 1:200 dilution.

GTX102376_IHC_19011506_858.jpg

GTX102376 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H1299 Xenograft , using NGFRAP1(GTX102376) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169