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NADE antibody [N1C3]

Anti-NADE antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102376
Anti-NADE antibody [N1C3] used in Western Blot (WB). GTX102376
Anti-NADE antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX102376

Cat. No. GTX102376

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all NADE products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

13 kDa. ( Note )

Positive Control

293T , A431 , H1299

Predict Reactivity

Mouse, Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.38 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NADE. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036339

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

brain expressed X-linked 3 , Bex , DXS6984E , HGR74 , NADE , NGFRAP1

Cellular Localization

Nucleus , Cytoplasm

Background

May be a signaling adapter molecule involved in p75NTR-mediated apoptosis induced by NGF. Plays a role in zinc-triggered neuronal death (By similarity). May play an important role in the pathogenesis of neurogenetic diseases.

Database

Research Area

DATA IMAGES

Anti-NADE antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102376

GTX102376 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NADE(GTX102376) antibody at 1:200 dilution.

Anti-NADE antibody [N1C3] used in Western Blot (WB). GTX102376

GTX102376 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: A431 (GTX27909)
C: H1299
15% SDS PAGE
GTX102376 diluted at 1:1000

Anti-NADE antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX102376

GTX102376 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H1299 Xenograft , using NGFRAP1(GTX102376) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169