Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma, using NAGLU(GTX114569) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NAGLU antibody [N3C1], Internal (GTX114569) diluted at 1:1000.

NAGLU antibody [N3C1], Internal detects NAGLU protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
NAGLU antibody [N3C1], Internal (GTX114569) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NAGLU antibody [N3C1], Internal (GTX114569) diluted at 1:1000.

NAGLU antibody [N3C1], Internal detects NAGLU protein at cytoplasm by immunofluorescent analysis.
Sample: U-87 MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NAGLU protein stained by NAGLU antibody [N3C1], Internal (GTX114569) diluted at 1:200.
Red: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody (GTX631836) diluted at 1:200.
Blue: Hoechst 33342 staining.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NAGLU antibody [N3C1], Internal (GTX114569) diluted at 1:1000.