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NAGLU antibody [N3C1], Internal

Anti-NAGLU antibody [N3C1], Internal used in Western Blot (WB). GTX114569
Anti-NAGLU antibody [N3C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114569
Anti-NAGLU antibody [N3C1], Internal used in Western Blot (WB). GTX114569
Anti-NAGLU antibody [N3C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX114569
Anti-NAGLU antibody [N3C1], Internal used in Western Blot (WB). GTX114569
Anti-NAGLU antibody [N3C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX114569

Cat. No. GTX114569

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all NAGLU products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

82 kDa. ( Note )

Positive Control

U87-MG , SK-N-SH , IMR32 , SK-N-AS , mouse brain , rat brain

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NAGLU. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10620563

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

N-acetyl-alpha-glucosaminidase , CMT2V , MPS-IIIB , MPS3B , NAG , UFHSD

Background

This gene encodes an enzyme that degrades heparan sulfate by hydrolysis of terminal N-acetyl-D-glucosamine residues in N-acetyl-alpha-D-glucosaminides. Defects in this gene are the cause of mucopolysaccharidosis type IIIB (MPS-IIIB), also known as Sanfilippo syndrome B. This disease is characterized by the lysosomal accumulation and urinary excretion of heparan sulfate. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-NAGLU antibody [N3C1], Internal used in Western Blot (WB). GTX114569

GTX114569 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NAGLU antibody [N3C1], Internal (GTX114569) diluted at 1:1000.

Anti-NAGLU antibody [N3C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114569

GTX114569 ICC/IF Image

NAGLU antibody [N3C1], Internal detects NAGLU protein at cytoplasm by immunofluorescent analysis.
Sample: U-87 MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NAGLU protein stained by NAGLU antibody [N3C1], Internal (GTX114569) diluted at 1:200.
Red: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody (GTX631836) diluted at 1:200.
Blue: Hoechst 33342 staining.
Anti-NAGLU antibody [N3C1], Internal used in Western Blot (WB). GTX114569

GTX114569 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NAGLU antibody [N3C1], Internal (GTX114569) diluted at 1:1000.

Anti-NAGLU antibody [N3C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX114569

GTX114569 IHC-P Image

NAGLU antibody [N3C1], Internal detects NAGLU protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
NAGLU antibody [N3C1], Internal (GTX114569) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NAGLU antibody [N3C1], Internal used in Western Blot (WB). GTX114569

GTX114569 WB Image

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NAGLU antibody [N3C1], Internal (GTX114569) diluted at 1:1000.

Anti-NAGLU antibody [N3C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX114569

GTX114569 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma, using NAGLU(GTX114569) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169