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NAGLU antibody

Anti-NAGLU antibody used in IHC (Paraffin sections) (IHC-P). GTX105504
Anti-NAGLU antibody used in IHC (Paraffin sections) (IHC-P). GTX105504
Anti-NAGLU antibody used in IHC (Paraffin sections) (IHC-P). GTX105504
Anti-NAGLU antibody used in IHC (Paraffin sections) (IHC-P). GTX105504
Anti-NAGLU antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105504
Anti-NAGLU antibody used in IHC (Frozen sections) (IHC-Fr). GTX105504
Anti-NAGLU antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105504
Anti-NAGLU antibody used in IHC (Paraffin sections) (IHC-P). GTX105504

Cat No. GTX105504

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application ICC/IF, IHC-P, IHC-Fr
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

82 kDa. ( Note )

Predict Reactivity

Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.69 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NAGLU. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

N-acetyl-alpha-glucosaminidase , CMT2V , MPS-IIIB , MPS3B , NAG , UFHSD

Background

This gene encodes an enzyme that degrades heparan sulfate by hydrolysis of terminal N-acetyl-D-glucosamine residues in N-acetyl-alpha-D-glucosaminides. Defects in this gene are the cause of mucopolysaccharidosis type IIIB (MPS-IIIB), also known as Sanfilippo syndrome B. This disease is characterized by the lysosomal accumulation and urinary excretion of heparan sulfate. [provided by RefSeq]

Database

DATA IMAGES
Anti-NAGLU antibody used in IHC (Paraffin sections) (IHC-P). GTX105504

GTX105504 IHC-P Image

NAGLU antibody detects NAGLU protein at cytoplasm in rat pancreas by immunohistochemical analysis.
Sample: Paraffin-embedded rat pancreas.
NAGLU antibody (GTX105504) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NAGLU antibody used in IHC (Paraffin sections) (IHC-P). GTX105504

GTX105504 IHC-P Image

NAGLU antibody detects NAGLU protein at cytoplasm in rat duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded rat duodenum.
NAGLU antibody (GTX105504) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NAGLU antibody used in IHC (Paraffin sections) (IHC-P). GTX105504

GTX105504 IHC-P Image

NAGLU antibody detects NAGLU protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Green: NAGLU antibody (GTX105504) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488) (GTX213110-04).
Blue: Nuclear staining with Hoechst 33342.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NAGLU antibody used in IHC (Paraffin sections) (IHC-P). GTX105504

GTX105504 IHC-P Image

NAGLU antibody detects NAGLU protein at cytoplasm in mouse pancreas by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
NAGLU antibody (GTX105504) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NAGLU antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105504

GTX105504 ICC/IF Image

NAGLU antibody detects NAGLU protein at cytoplasm by immunofluorescent analysis.
Sample: U-87 MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NAGLU protein stained by NAGLU antibody (GTX105504) diluted at 1:250.
Blue: Hoechst 33342 staining.

Anti-NAGLU antibody used in IHC (Frozen sections) (IHC-Fr). GTX105504

GTX105504 IHC-Fr Image

NAGLU antibody detects NAGLU Protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: NAGLU stained by NAGLU antibody (GTX105504) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

Anti-NAGLU antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105504

GTX105504 ICC/IF Image

NAGLU antibody detects NAGLU protein at lysosome by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NAGLU protein stained by NAGLU antibody (GTX105504) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-NAGLU antibody used in IHC (Paraffin sections) (IHC-P). GTX105504

GTX105504 IHC-P Image

NAGLU antibody detects NAGLU protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
NAGLU antibody (GTX105504) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169