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NAGLU antibody

Anti-NAGLU antibody used in IHC (Paraffin sections) (IHC-P). GTX105504
Anti-NAGLU antibody used in IHC (Paraffin sections) (IHC-P). GTX105504
Anti-NAGLU antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105504
Anti-NAGLU antibody used in IHC (Paraffin sections) (IHC-P). GTX105504
Anti-NAGLU antibody used in IHC (Paraffin sections) (IHC-P). GTX105504
Anti-NAGLU antibody used in IHC (Paraffin sections) (IHC-P). GTX105504
Anti-NAGLU antibody used in IHC (Frozen sections) (IHC-Fr). GTX105504
Anti-NAGLU antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105504

Cat. No. GTX105504

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all NAGLU products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

82 kDa. ( Note )

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.69 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NAGLU. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950971

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

N-acetyl-alpha-glucosaminidase , CMT2V , MPS-IIIB , MPS3B , NAG , UFHSD

Background

This gene encodes an enzyme that degrades heparan sulfate by hydrolysis of terminal N-acetyl-D-glucosamine residues in N-acetyl-alpha-D-glucosaminides. Defects in this gene are the cause of mucopolysaccharidosis type IIIB (MPS-IIIB), also known as Sanfilippo syndrome B. This disease is characterized by the lysosomal accumulation and urinary excretion of heparan sulfate. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-NAGLU antibody used in IHC (Paraffin sections) (IHC-P). GTX105504

GTX105504 IHC-P Image

NAGLU antibody detects NAGLU protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
NAGLU antibody (GTX105504) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NAGLU antibody used in IHC (Paraffin sections) (IHC-P). GTX105504

GTX105504 IHC-P Image

NAGLU antibody detects NAGLU protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Green: NAGLU antibody (GTX105504) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488) (GTX213110-04).
Blue: Nuclear staining with Hoechst 33342.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NAGLU antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105504

GTX105504 ICC/IF Image

NAGLU antibody detects NAGLU protein at cytoplasm by immunofluorescent analysis.
Sample: U-87 MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NAGLU protein stained by NAGLU antibody (GTX105504) diluted at 1:250.
Blue: Hoechst 33342 staining.
Anti-NAGLU antibody used in IHC (Paraffin sections) (IHC-P). GTX105504

GTX105504 IHC-P Image

NAGLU antibody detects NAGLU protein at cytoplasm in rat pancreas by immunohistochemical analysis.
Sample: Paraffin-embedded rat pancreas.
NAGLU antibody (GTX105504) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NAGLU antibody used in IHC (Paraffin sections) (IHC-P). GTX105504

GTX105504 IHC-P Image

NAGLU antibody detects NAGLU protein at cytoplasm in rat duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded rat duodenum.
NAGLU antibody (GTX105504) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NAGLU antibody used in IHC (Paraffin sections) (IHC-P). GTX105504

GTX105504 IHC-P Image

NAGLU antibody detects NAGLU protein at cytoplasm in mouse pancreas by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
NAGLU antibody (GTX105504) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NAGLU antibody used in IHC (Frozen sections) (IHC-Fr). GTX105504

GTX105504 IHC-Fr Image

NAGLU antibody detects NAGLU Protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: NAGLU stained by NAGLU antibody (GTX105504) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

Anti-NAGLU antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105504

GTX105504 ICC/IF Image

NAGLU antibody detects NAGLU protein at lysosome by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NAGLU protein stained by NAGLU antibody (GTX105504) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169