APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:1000-1:3000 |
| 1:50-1:200 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Predict Reactivity
Zebrafish, Rabbit, Sheep, Bovine, Dog, Chicken, Pig, Horse, Xenopus(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 150mM NaCl, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthesized peptide derived from human TBK1 around the phosphorylation site of Ser172.
Purification
Purified by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns
From serum
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
TANK binding kinase 1 , FTDALS4 , IIAE8 , NAK , T2K
Cellular Localization
Cytoplasm
Background
The NF-kappa-B (NFKB) complex of proteins is inhibited by I-kappa-B (IKB) proteins, which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the IKB proteins by IKB kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation and nuclear translocation of the NFKB complex. The protein encoded by this gene is similar to IKB kinases and can mediate NFKB activation in response to certain growth factors. [provided by RefSeq, Oct 2010]
Database
Research Area
DATA IMAGES
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GTX02815 WB Image
WB analysis of PMA treated HeLa whole cell lysates using GTX02815 NAK / TBK1 (phospho Ser172) antibody in the absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).
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GTX02815 IHC-P Image
IHC-P analysis of rat stomach tissue using GTX02815 NAK / TBK1 (phospho Ser172) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:100
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GTX02815 IHC-P Image
IHC-P analysis of rat brain tissue using GTX02815 NAK / TBK1 (phospho Ser172) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:100
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GTX02815 IHC-P Image
IHC-P analysis of rat colon tissue using GTX02815 NAK / TBK1 (phospho Ser172) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:100
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GTX02815 IHC-P Image
IHC-P analysis of human mammary cancer tissue using GTX02815 NAK / TBK1 (phospho Ser172) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:100
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GTX02815 IHC-P Image
IHC-P analysis of human tonsil tissue using GTX02815 NAK / TBK1 (phospho Ser172) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:200
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GTX02815 IHC-P Image
IHC-P analysis of mouse testis tissue using GTX02815 NAK / TBK1 (phospho Ser172) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:100
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GTX02815 IHC-P Image
IHC-P analysis of mouse spleen tissue using GTX02815 NAK / TBK1 (phospho Ser172) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:100
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GTX02815 IHC-P Image
IHC-P analysis of mouse kidney tissue using GTX02815 NAK / TBK1 (phospho Ser172) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:200
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GTX02815 IHC-P Image
IHC-P analysis of human lung cancer tissue using GTX02815 NAK / TBK1 (phospho Ser172) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:100
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GTX02815 ELISA Image
ELISA analysis of GTX02815 showing the specificity to NAK / TBK1 phospho Ser172 peptide
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide
Peptides concentration : 1μg/ml
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GTX02815 IHC-P Image
IHC-P analysis of rat lung tissue using GTX02815 NAK / TBK1 (phospho Ser172) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:200
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GTX02815 IHC-P Image
IHC-P analysis of mouse stomach tissue using GTX02815 NAK / TBK1 (phospho Ser172) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:100
|
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GTX02815 IHC-P Image
IHC-P analysis of mouse kidney tissue using GTX02815 NAK / TBK1 (phospho Ser172) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:100
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GTX02815 IHC-P Image
IHC-P analysis of rat testis tissue using GTX02815 NAK / TBK1 (phospho Ser172) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:100
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GTX02815 IHC-P Image
IHC-P analysis of mouse ovary tissue using GTX02815 NAK / TBK1 (phospho Ser172) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:100
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REFERENCE
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REVIEW
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