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NAK / TBK1 antibody [108A429]

Overexpression
Anti-NAK / TBK1 antibody [108A429] used in Western Blot (WB). GTX12116
Anti-NAK / TBK1 antibody [108A429] used in IHC (Paraffin sections) (IHC-P). GTX12116
Anti-NAK / TBK1 antibody [108A429] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX12116
Anti-NAK / TBK1 antibody [108A429] used in Western Blot (WB). GTX12116
Anti-NAK / TBK1 antibody [108A429] used in Western Blot (WB). GTX12116

Cat. No. GTX12116

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 108A429

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Bovine, Dog
Package
50 μg ($399)
See all NAK / TBK1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/ml
ICC/IF 1:20
IHC-P 1:20 - 1:1000
Not tested in other applications.

Calculated MW

84 kDa. ( Note )

Predict Reactivity

Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to amino acids 563-577 (YYQFKKDKAERRLAYC) of human NAK / TBK1 was used as immunogen for this antibody (NP_037386).

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_370539

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

TANK binding kinase 1 , FTDALS4 , IIAE8 , NAK , T2K

Cellular Localization

Cytoplasm

Background

The NF-kappa-B (NFKB) complex of proteins is inhibited by I-kappa-B (IKB) proteins, which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the IKB proteins by IKB kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation and nuclear translocation of the NFKB complex. The protein encoded by this gene is similar to IKB kinases and can mediate NFKB activation in response to certain growth factors. For example, the protein can form a complex with the IKB protein TANK and TRAF2 and release the NFKB inhibition caused by TANK. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-NAK / TBK1 antibody [108A429] used in Western Blot (WB). GTX12116

GTX12116 WB Image

Overexpression

WB analysis of HEK293 mock cells (Lane 1) or overexpressing human NAK / TBK1 using GTX12116 NAK / TBK1 antibody [108A429].
Dilution : 2 μg/ml

Anti-NAK / TBK1 antibody [108A429] used in IHC (Paraffin sections) (IHC-P). GTX12116

GTX12116 IHC-P Image

IHC-P analysis of human testis tissue using GTX12116 NAK / TBK1 antibody [108A429].

Anti-NAK / TBK1 antibody [108A429] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX12116

GTX12116 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX12116 NAK / TBK1 antibody [108A429].
Green : primary antibody
Red : Tubulin
Blue : DAPI
Dilution : 1:10

Anti-NAK / TBK1 antibody [108A429] used in Western Blot (WB). GTX12116

GTX12116 WB Image

WB analysis of Daudi (left) and Raw 264.7 (right) cell lysate using GTX12116 NAK / TBK1 antibody [108A429].

Anti-NAK / TBK1 antibody [108A429] used in Western Blot (WB). GTX12116

GTX12116 WB Image

WB analysis of Daudi (A) and RAW264.7 (B) cell lysate using GTX12116 NAK / TBK1 antibody [108A429].
Dilution : 2 μg/ml

REFERENCE

Application Reference

Zhao B et al. Nature 2019; 569 (7758) : 718-722 A conserved PLPLRT/SD motif of STING mediates the recruitment and activation of TBK1.
Application : ICC/IF
Reactivity : Human
Ogasawara N et al. J Clin Biochem Nutr 2011; 48 (2) : 154-60 Rebamipide suppresses TLR-TBK1 signaling pathway resulting in regulating IRF3/7 and IFN-α/β reduction.
Application : ICC/IF
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399