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NAK antibody [108A429]

Anti-NAK antibody [108A429] used in Western Blot (WB). GTX12116
Anti-NAK antibody [108A429] used in Western Blot (WB). GTX12116
Anti-NAK antibody [108A429] used in IHC (Paraffin sections) (IHC-P). GTX12116
Anti-NAK antibody [108A429] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX12116
Anti-NAK antibody [108A429] used in Western Blot (WB). GTX12116

Cat No. GTX12116

Host Mouse
Clonality Monoclonal
Clone Name 108A429
Isotype IgG1
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat, Bovine, Dog
Package
50 μg ($289)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 - 2 μg/ml
ICC/IF 1:20
IHC-P 1:20 - 1:1000
Not tested in other applications.

Calculated MW

84 kDa. ( Note )

Predict Reactivity

Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to amino acids 563-577 (YYQFKKDKAERRLAYC) of human TBK1 was used as immunogen for this antibody (NP_037386).

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Tank Binding Kinase 1,Ftdals4,Iiae8,Nak,T2K,Tbk1

Cellular Localization

Cytoplasm

Background

The NF-kappa-B (NFKB) complex of proteins is inhibited by I-kappa-B (IKB) proteins, which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the IKB proteins by IKB kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation and nuclear translocation of the NFKB complex. The protein encoded by this gene is similar to IKB kinases and can mediate NFKB activation in response to certain growth factors. For example, the protein can form a complex with the IKB protein TANK and TRAF2 and release the NFKB inhibition caused by TANK. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-NAK antibody [108A429] used in Western Blot (WB). GTX12116

GTX12116 WB Image

WB analysis of Daudi (left) and Raw 264.7 (right) cell lysate using GTX12116 NAK antibody [108A429].

Anti-NAK antibody [108A429] used in Western Blot (WB). GTX12116

GTX12116 WB Image

WB analysis of Daudi (A) and RAW264.7 (B) cell lysate using GTX12116 NAK antibody [108A429].
Dilution : 2 μg/ml

Anti-NAK antibody [108A429] used in IHC (Paraffin sections) (IHC-P). GTX12116

GTX12116 IHC-P Image

IHC-P analysis of human testis tissue using GTX12116 NAK antibody [108A429].

Anti-NAK antibody [108A429] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX12116

GTX12116 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX12116 NAK antibody [108A429].
Green : primary antibody
Red : Tubulin
Blue : DAPI
Dilution : 1:10

Anti-NAK antibody [108A429] used in Western Blot (WB). GTX12116

GTX12116 WB Image

WB analysis of HEK293 mock cells (Lane 1) or overexpressing human NAK using GTX12116 NAK antibody [108A429].
Dilution : 2 μg/ml

REFERENCE
REVIEW
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Package List Price ($)
$ 289