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NANS antibody [AT1G6]

Anti-NANS antibody [AT1G6] used in Western Blot (WB). GTX53749

Cat No. GTX53749

Host

Mouse

Clonality

Monoclonal

Clone Name

AT1G6

Isotype

IgG1

Application

WB, ELISA

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1,000.

Calculated MW

40 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human NANS (1-359aa) purified from E. coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

N-Acetylneuraminate Synthase , Hel-S-100 , Sas , Semdcg , Semdg , Nans

Background

This gene encodes an enzyme that functions in the biosynthetic pathways of sialic acids. In vitro, the encoded protein uses N-acetylmannosamine 6-phosphate and mannose 6-phosphate as substrates to generate phosphorylated forms of N-acetylneuraminic acid (Neu5Ac) and 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN), respectively; however, it exhibits much higher activity toward the Neu5Ac phosphate product. In insect cells, expression of this gene results in Neu5Ac and KDN production. This gene is related to the E. coli sialic acid synthase gene neuB, and it can partially restore sialic acid synthase activity in an E. coli neuB-negative mutant. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-NANS antibody [AT1G6] used in Western Blot (WB). GTX53749

GTX53749 WB Image

WB analysis of HeLa lysate (40ug) using NANS antibody at the indicated dilutions.

REFERENCE

There are currently no references for NANS antibody [AT1G6] (GTX53749). Be the first to share your publications with this product.

REVIEW

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SDS
Sodium Azide.pdf