GeneTex
United States (US)

NANS antibody [N1C3]

GTX105223_IHC_19011506_937.jpg
GTX105223_WB_19011506_165.jpg
GTX105223_39862_20141205_IFA_19011506_601.jpg

Cat No. GTX105223

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

A431 , HeLa , HepG2

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.21mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NANS. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

SAS antibody, NANS antibody, sialic acid synthase antibody, N-acetylneuraminate synthase antibody, N-acetylneuraminic acid phosphate synthase antibody, N-acetylneuraminate-9-phosphate synthase antibody, sialic acid phosphate synthase antibody, N-acetylneuraminic acid synthase antibody

Background

This gene encodes an enzyme that functions in the biosynthetic pathways of sialic acids. In vitro, the encoded protein uses N-acetylmannosamine 6-phosphate and mannose 6-phosphate as substrates to generate phosphorylated forms of N-acetylneuraminic acid (Neu5Ac) and 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN), respectively; however, it exhibits much higher activity toward the Neu5Ac phosphate product. In insect cells, expression of this gene results in Neu5Ac and KDN production. This gene is related to the E. coli sialic acid synthase gene neuB, and it can partially restore sialic acid synthase activity in an E. coli neuB-negative mutant. [provided by RefSeq]
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Database

DATA IMAGES
GTX105223_IHC_19011506_937.jpg

GTX105223 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human normal gastric epithelium (gland), using NANS(GTX105223) antibody at 1:100 dilution.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX105223_WB_19011506_165.jpg

GTX105223 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
10% SDS PAGE
NANS antibody
GTX105223 diluted at 1:1000

GTX105223_39862_20141205_IFA_19011506_601.jpg

GTX105223 ICC/IF Image

NANS antibody [N1C3] detects NANS protein at nucleus by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NANS protein stained by NANS antibody [N1C3] (GTX105223) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169