*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
A431 , HeLa , HepG2
Mouse, Rat, Bovine, Chicken, Pig(>80% identity)
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Recombinant protein encompassing a sequence within the center region of human NANS. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
SAS antibody, NANS antibody, sialic acid synthase antibody, N-acetylneuraminate synthase antibody, N-acetylneuraminic acid phosphate synthase antibody, N-acetylneuraminate-9-phosphate synthase antibody, sialic acid phosphate synthase antibody, N-acetylneuraminic acid synthase antibody
This gene encodes an enzyme that functions in the biosynthetic pathways of sialic acids. In vitro, the encoded protein uses N-acetylmannosamine 6-phosphate and mannose 6-phosphate as substrates to generate phosphorylated forms of N-acetylneuraminic acid (Neu5Ac) and 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN), respectively; however, it exhibits much higher activity toward the Neu5Ac phosphate product. In insect cells, expression of this gene results in Neu5Ac and KDN production. This gene is related to the E. coli sialic acid synthase gene neuB, and it can partially restore sialic acid synthase activity in an E. coli neuB-negative mutant. [provided by RefSeq]
GTX105223 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human normal gastric epithelium (gland), using NANS(GTX105223) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
GTX105223 WB Image
Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
10% SDS PAGE
GTX105223 diluted at 1:1000
GTX105223 ICC/IF Image
NANS antibody [N1C3] detects NANS protein at nucleus by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NANS protein stained by NANS antibody [N1C3] (GTX105223) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.