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NANS antibody

Anti-NANS antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118242
Anti-NANS antibody used in Western Blot (WB). GTX118242
Anti-NANS antibody used in IHC (Paraffin sections) (IHC-P). GTX118242

Cat. No. GTX118242

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all NANS products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

HepG2

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NANS. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619740

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

N-acetylneuraminate synthase , HEL-S-100 , SAS , SEMDCG , SEMDG

Background

This gene encodes an enzyme that functions in the biosynthetic pathways of sialic acids. In vitro, the encoded protein uses N-acetylmannosamine 6-phosphate and mannose 6-phosphate as substrates to generate phosphorylated forms of N-acetylneuraminic acid (Neu5Ac) and 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN), respectively; however, it exhibits much higher activity toward the Neu5Ac phosphate product. In insect cells, expression of this gene results in Neu5Ac and KDN production. This gene is related to the E. coli sialic acid synthase gene neuB, and it can partially restore sialic acid synthase activity in an E. coli neuB-negative mutant. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-NANS antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118242

GTX118242 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NANS(GTX118242) antibody at 1:500 dilution.

Anti-NANS antibody used in Western Blot (WB). GTX118242

GTX118242 WB Image

Sample (30 ug of whole cell lysate)
A: HepG2
10% SDS PAGE
GTX118242 diluted at 1:5000

Anti-NANS antibody used in IHC (Paraffin sections) (IHC-P). GTX118242

GTX118242 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using NANS(GTX118242) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169