GeneTex
United States (US)

NAP2 antibody

Cat No. GTX29554

Host Rabbit
Clonality Polyclonal
Application WB, ELISA, Neut
Reactivity Human
APPLICATION

Application Note

Neutralization:To yield one-half maximal inhibition [ND50] of the biological activity of hNAP-2 (50 ng/ml), a concentration of 1.75 - 3.0 μg/ml of this antibody is required. ELISA:To detect hNAP-2 by direct ELISA (using 100μl/well antibody solution) a concentration of at least 0.5μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hNAP-2. Western Blot:To detect hNAP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNAP-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Calculated MW

14 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS, pH 7.4, no preservative, sterile filtered

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Antigen Species

Human

Immunogen

Highly pure (>98%) recombinant hNAP-2 (human Neutrophil Activating Protein-2).

Purification

Immunogen affinity purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Pro-Platelet Basic Protein,B-Tg1,Beta-Tg,Ctap-Iii,Ctap3,Ctapiii,Cxcl7,La-Pf4,Ldgf,Mdgf,Nap-2,Pbp,Scyb7,Tc1,Tc2,Tgb,Tgb1,Thbgb,Thbgb1,Ppbp

Cellular Localization

Secreted

Background

NAP2 is a platelet-derived growth factor that belongs to the CXC chemokine family. This growth factor is a potent chemoattractant and activator of neutrophils. It has been shown to stimulate various cellular processes including DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and sythesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by synovial cells.

Database

Package List Price ($)
$ 339