GeneTex

  • Country / Location Selection

United States (US)

NARF antibody

Anti-NARF antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115913
Anti-NARF antibody used in IHC (Paraffin sections) (IHC-P). GTX115913
Anti-NARF antibody used in Western Blot (WB). GTX115913
Anti-NARF antibody used in Western Blot (WB). GTX115913

Cat. No. GTX115913

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all NARF products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Positive Control

mouse muscle , A549 , H1299 , HCT116 , MCF-7

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NARF. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11169811

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nuclear prelamin A recognition factor , IOP2

Cellular Localization

Nucleus

Background

Several proteins have been found to be prenylated and methylated at their carboxyl-terminal ends. Prenylation was initially believed to be important only for membrane attachment. However, another role for prenylation appears to be its importance in protein-protein interactions. The only nuclear proteins known to be prenylated in mammalian cells are prelamin A- and B-type lamins. Prelamin A is farnesylated and carboxymethylated on the cysteine residue of a carboxyl-terminal CaaX motif. This post-translationally modified cysteine residue is removed from prelamin A when it is endoproteolytically processed into mature lamin A. The protein encoded by this gene binds to the prenylated prelamin A carboxyl-terminal tail domain. It may be a component of a prelamin A endoprotease complex. The encoded protein is located in the nucleus, where it partially colocalizes with the nuclear lamina. It shares limited sequence similarity with iron-only bacterial hydrogenases. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene, including one with a novel exon that is generated by RNA editing. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-NARF antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115913

GTX115913 ICC/IF Image

NARF antibody detects NARF protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NARF protein stained by NARF antibody (GTX115913) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-NARF antibody used in IHC (Paraffin sections) (IHC-P). GTX115913

GTX115913 IHC-P Image

Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using NARF(GTX115913) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NARF antibody used in Western Blot (WB). GTX115913

GTX115913 WB Image

Sample (50 ug of whole cell lysate)
A: mouse muscle
7.5% SDS PAGE
GTX115913 diluted at 1:1000

Anti-NARF antibody used in Western Blot (WB). GTX115913

GTX115913 WB Image

Sample (30 ug of whole cell lysate)
A: A549
B: H1299
C: HCT116
D: MCF-7
7.5% SDS PAGE
GTX115913 diluted at 1:1000

REFERENCE

There are currently no references for NARF antibody (GTX115913). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for NARF antibody (GTX115913). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169