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NAT10 antibody [N1N2], N-term

Anti-NAT10 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX119166
Anti-NAT10 antibody [N1N2], N-term used in Western Blot (WB). GTX119166
Anti-NAT10 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119166
Anti-NAT10 antibody [N1N2], N-term used in Western Blot (WB). GTX119166

Cat. No. GTX119166

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all NAT10 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

116 kDa. ( Note )

Positive Control

MCF-7

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human NAT10. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618723

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

N-acetyltransferase 10 , ALP , Kre33 , NET43

Cellular Localization

Nucleus , nucleolus

Background

Has protein acetyltransferase activity in vitro. Can acetylate both histones and microtubules. Histone acetylation may regulate transcription and mitotic chromosome de-condensation. Activates telomerase activity by stimulating the transcription of TERT, and may also regulate telomerase function by affecting the balance of telomerase subunit assembly, disassembly, and localization. Acetylates alpha-tubulin, which may affect microtubule stability and cell division.

Database

Research Area

DATA IMAGES

Anti-NAT10 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX119166

GTX119166 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SCC4 xenograft, using NAT10(GTX119166) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NAT10 antibody [N1N2], N-term used in Western Blot (WB). GTX119166

GTX119166 WB Image

Sample (30 ug of whole cell lysate)
A: MCF-7
7.5% SDS PAGE
GTX119166 diluted at 1:1000

Anti-NAT10 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119166

GTX119166 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed MCF-7, using NAT10(GTX119166) antibody at 1:200 dilution.

Anti-NAT10 antibody [N1N2], N-term used in Western Blot (WB). GTX119166

GTX119166 WB Image

The data was published in the journal PLoS One in 2013.PMID: 24260471

REFERENCE

Application Reference

Alsarraj J et al. PLoS One 2013; 8 (11) : e80746 BRD4 short isoform interacts with RRP1B, SIPA1 and components of the LINC complex at the inner face of the nuclear membrane.
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169