Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NAT10 antibody (GTX119167) diluted at 1:1000.

Various tissue extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NAT10 antibody (GTX119167) diluted at 1:500.

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using NAT10 antibody (GTX119167) at 1:300 dilution.