GeneTex
United States (US)

NAT5 antibody

GTX32736_20180530_WB_18121410_978.jpg
GTX32736_20180530_IP_18121410_504.jpg
GTX32736_20180530_IHC-P_18121410_588.jpg
GTX32736_20180530_ICCIF_18121410_982.jpg

Cat No. GTX32736

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:100
IHC-P 1:50 - 1:200
IP Assay dependent
Not tested in other applications.

Calculated MW

20 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 49-178 of human NAA20 (NP_057184.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

N(Alpha)-Acetyltransferase 20, Natb Catalytic Subunit,Nat3,Nat3P,Nat5,Nat5P,Dj1002M8.1,Naa20

Background

NAT5 is a component of N-acetyltransferase complex B (NatB). Human NatB performs cotranslational N(alpha)-terminal acetylation of methionine residues when they are followed by asparagine (Starheim et al., 2008 [PubMed 18570629]).[supplied by OMIM, Apr 2009]

Database

DATA IMAGES
GTX32736_20180530_WB_18121410_978.jpg

GTX32736 WB Image

WB analysis of various samples using GTX32736 NAT5 antibody.The signal was developed with ECL plus-Enhanced.
Dilution : 1:1000
Loading : 25μg per lane

GTX32736_20180530_IP_18121410_504.jpg

GTX32736 IP Image

IP analysis of MCF7 cell lysate using GTX32736 NAT5 antibody.
IP reaction : 1μg antibody / 200μg cell lysate
Dilution : 1:1000

GTX32736_20180530_IHC-P_18121410_588.jpg

GTX32736 IHC-P Image

IHC-P analysis of human oophoroma tissue using GTX32736 NAT5 antibody.
Dilution : 1:100

GTX32736_20180530_ICCIF_18121410_982.jpg

GTX32736 ICC/IF Image

ICC/IF analysis of A549 cell using GTX32736 NAT5 antibody.
Blue : DAPI

Package List Price ($)
$ 329