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NBR1 antibody

Anti-NBR1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114539
Anti-NBR1 antibody used in Western Blot (WB). GTX114539
Anti-NBR1 antibody used in Western Blot (WB). GTX114539

Cat No. GTX114539

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

107 kDa. ( Note )

Positive Control

Jurkat , NIH-3T3 , JC

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NBR1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11164842

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

NBR1 autophagy cargo receptor , 1A1-3B , IAI3B , M17S2 , MIG19

Background

The protein encoded by this gene was originally identified as an ovarian tumor antigen monitored in ovarian cancer. The encoded protein contains a B-box/coiled coil motif, which is present in many genes with transformation potential, but the function of this protein is unknown. This gene is located on a region of chromosome 17q21.1 that is in close proximity to tumor suppressor gene BRCA1. Three alternatively spliced variants encoding the same protein have been identified for this gene. [provided by RefSeq]

Database

DATA IMAGES

Anti-NBR1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114539

GTX114539 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using NBR1(GTX114539) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NBR1 antibody used in Western Blot (WB). GTX114539

GTX114539 WB Image

Sample (30 μg of whole cell lysate)
A: Jurkat
7.5% SDS PAGE
GTX114539 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NBR1 antibody used in Western Blot (WB). GTX114539

GTX114539 WB Image

Sample (30 μg of whole cell lysate)
A: NIH-3T3
B: JC
7.5% SDS PAGE
GTX114539 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169