Recommended Starting Dilutions:
For IP: Use at an assay dependent dilution.
For ICC/IF: Use at a dilution of 1:100.
For WB: 1:500-1:3000. Predicted molecular weight: 91 kDa.
Optimal dilutions/concentrations should be determined by the researcher.
293T , MCF7 , HeLa , Raji
Phosphate-buffered saline, pH 7.4, containing no preservatives
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
The complete coding region of the human p95/NBS1 expressed in E. coli.
Protein G purified
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Nijmegen breakage syndrome (NBS, nibrin) is an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The 1C3 antibody recognizes the 95 kDa NBS protein which contains a fork FHA head-associated domain that is adjacent to BRCT, a breast cancer C terminal domain, that has been implicated in protein-protein interactions. NBS is member of the Mre11/Rad50, p400 double-strand break repair complex involved in double-strand break repair and cell-cycle checkpoint functions.