GeneTex
United States (US)

NBS1 antibody [1C3]

KO/KD
GTX70222_40004_20151112_WB_18121411_519.jpg
GTX70222_40004_20161103_WB_shRNA_watermark_18121411_398.jpg
NBS1-antibody-1C3-GTX70222-ICC_IF-1_18121411_745.jpg
NBS1-antibody-1C3-GTX70222-WB-2_18121411_912.jpg

Cat No. GTX70222

Host Mouse
Clonality Monoclonal
Clone Name 1C3
Isotype IgG1
Application WB, ICC/IF, IP
Reactivity Human, Mouse
APPLICATION

Application Note

Recommended Starting Dilutions:
For IP: Use at an assay dependent dilution.
For ICC/IF: Use at a dilution of 1:100.
For WB: 1:500-1:3000. Predicted molecular weight: 91 kDa.
Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

95 kDa. ( Note )

Positive Control

293T , MCF7 , HeLa , Raji
PROPERTIES

Form

Liquid

Buffer

Phosphate-buffered saline, pH 7.4, containing no preservatives

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The complete coding region of the human p95/NBS1 expressed in E. coli.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

NBS p95 nibrin nibrin breakage syndrome MRN hNBS1 hNBS

Background

Nijmegen breakage syndrome (NBS, nibrin) is an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The 1C3 antibody recognizes the 95 kDa NBS protein which contains a fork FHA head-associated domain that is adjacent to BRCT, a breast cancer C terminal domain, that has been implicated in protein-protein interactions. NBS is member of the Mre11/Rad50, p400 double-strand break repair complex involved in double-strand break repair and cell-cycle checkpoint functions.

Database

Research Area

DATA IMAGES
GTX70222_40004_20151112_WB_18121411_519.jpg

GTX70222 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NBS1 antibody [1C3] (GTX70222) diluted at1:500.

GTX70222_40004_20161103_WB_shRNA_watermark_18121411_398.jpg

GTX70222 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NBS1 antibody [1C3] (GTX70222) diluted at 1:500.

NBS1-antibody-1C3-GTX70222-ICC_IF-1_18121411_745.jpg

GTX70222 ICC/IF Image

Immunofluorescence analysis of HeLa, using NBS1(GTX70222) antibody at 1:100 dilution.

NBS1-antibody-1C3-GTX70222-WB-2_18121411_912.jpg

GTX70222 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
7.5% SDS PAGE
GTX70222 diluted at 1:1000

REFERENCE
Application Reference