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NBS1 antibody [1D7]

Anti-NBS1 antibody [1D7] used in Western Blot (WB). GTX70224
Anti-NBS1 antibody [1D7] used in Western Blot (WB). GTX70224
Anti-NBS1 antibody [1D7] used in Western Blot (WB). GTX70224
Anti-NBS1 antibody [1D7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70224

Cat No. GTX70224

Host Mouse
Clonality Monoclonal
Clone Name 1D7
Isotype IgG1
Application WB, ICC/IF, IP
Reactivity Human, Mouse, Rat
Package
100 μl ($319)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100
IP Assay dependent
Not tested in other applications.

Calculated MW

95 kDa. ( Note )

Positive Control

293T , MCF7 , HeLa (NE) , Raji
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The complete coding region of the human p95/NBS1 expressed in E. coli.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Nibrin,At-V1,At-V2,Atv,Nbs,Nbs1,P95,Nbn

Background

Nijmegen breakage syndrome (NBS, nibrin) is an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The 1C3 antibody recognizes the 95 kDa NBS protein which contains a fork FHA head-associated domain that is adjacent to BRCT, a breast cancer C terminal domain, that has been implicated in protein-protein interactions. NBS is member of the Mre11/Rad50, p400 double-strand break repair complex involved in double-strand break repair and cell-cycle checkpoint functions.

Database

Research Area

DATA IMAGES
Anti-NBS1 antibody [1D7] used in Western Blot (WB). GTX70224

GTX70224 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NBS1 antibody [1D7] (GTX70224) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-NBS1 antibody [1D7] used in Western Blot (WB). GTX70224

GTX70224 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NBS1 antibody [1D7] (GTX70224) diluted at1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-NBS1 antibody [1D7] used in Western Blot (WB). GTX70224

GTX70224 WB Image

Sample (30 µg of whole cell lysate)
A: HepG2 (GTX27900)
7.5% SDS PAGE
GTX70224 diluted at 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-NBS1 antibody [1D7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70224

GTX70224 ICC/IF Image

Immunofluorescence analysis of HeLa, using NBS1(GTX70224) antibody at 1:100 dilution.

REFERENCE
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Application Reference
Package List Price ($)
$ 319