GeneTex
United States (US)

NBS1 antibody

KO/KD
GTX103229_41101_20161103_WB_shRNA_watermark_19011506_222.jpg
GTX103229_41101_IFA_19011506_822.jpg
GTX103229_41101_WB_19011506_516.jpg
GTX103229_41101_IP_19011506_283.jpg
GTX103229_41101_20151126_WB_19011506_813.jpg

Cat No. GTX103229

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IP
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

85 kDa. ( Note )

Positive Control

293T , NT2D1

Predict Reactivity

Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NBS1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Nibrin,At-V1,At-V2,Atv,Nbs,Nbs1,P95,Nbn

Cellular Localization

Nucleus , Telomere

Background

Mutations in this gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX103229_41101_20161103_WB_shRNA_watermark_19011506_222.jpg

GTX103229 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NBS1 antibody (GTX103229) diluted at 1:2000.

GTX103229_41101_IFA_19011506_822.jpg

GTX103229 ICC/IF Image

NBS1 antibody detects NBS1 protein at nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NBS1 protein stained by NBS1 antibody (GTX103229) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX103229_41101_WB_19011506_516.jpg

GTX103229 WB Image

NBS1 antibody detects NBS1 protein by western blot analysis.
A. 30 μg NT2D1 whole cell extract
7.5 % SDS-PAGE
NBS1 antibody (GTX103229) dilution: 1:5000

GTX103229_41101_IP_19011506_283.jpg

GTX103229 IP Image

NBS1 antibody immunoprecipitates nibrin protein in IP experiments.
IP samples: NT2D1 whole cell extract
A. 50 μg NT2D1 whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of nibrin protein by 4 μg NBS1 antibody (GTX103229)
7.5 % SDS-PAGE
The immunoprecipitated nibrin protein was detected by NBS1 antibody (GTX103229) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

GTX103229_41101_20151126_WB_19011506_813.jpg

GTX103229 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NBS1 antibody (GTX103229) diluted at 1:2000.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf