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NBS1 antibody

KO/KD
Anti-NBS1 antibody used in Western Blot (WB). GTX103229
Anti-NBS1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103229
Anti-NBS1 antibody used in Western Blot (WB). GTX103229
Anti-NBS1 antibody used in Immunoprecipitation (IP). GTX103229
Anti-NBS1 antibody used in Western Blot (WB). GTX103229

Cat. No. GTX103229

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all NBS1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

85 kDa. ( Note )

Positive Control

NT2D1 , 293T , A431 , HeLa , HepG2 , 293T

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NBS1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2616070

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nibrin , AT-V1 , AT-V2 , ATV , NBS , NBS1 , P95

Cellular Localization

Nucleus , Telomere

Background

Mutations in this gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-NBS1 antibody used in Western Blot (WB). GTX103229

GTX103229 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NBS1 antibody (GTX103229) diluted at 1:2000.

Anti-NBS1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103229

GTX103229 ICC/IF Image

NBS1 antibody detects NBS1 protein at nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NBS1 stained by NBS1 antibody (GTX103229) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.

Anti-NBS1 antibody used in Western Blot (WB). GTX103229

GTX103229 WB Image

NBS1 antibody detects NBS1 protein by western blot analysis.
A. 30 μg NT2D1 whole cell extract
7.5 % SDS-PAGE
NBS1 antibody (GTX103229) dilution: 1:5000

Anti-NBS1 antibody used in Immunoprecipitation (IP). GTX103229

GTX103229 IP Image

NBS1 antibody immunoprecipitates nibrin protein in IP experiments.
IP samples: NT2D1 whole cell extract
A. 50 μg NT2D1 whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of nibrin protein by 4 μg NBS1 antibody (GTX103229)
7.5 % SDS-PAGE
The immunoprecipitated nibrin protein was detected by NBS1 antibody (GTX103229) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Anti-NBS1 antibody used in Western Blot (WB). GTX103229

GTX103229 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NBS1 antibody (GTX103229) diluted at 1:2000.

REFERENCE

Application Reference

Dubich T et al. J Mol Med (Berl) 2021; 99 (3) : 425-438 3D culture conditions support Kaposi's sarcoma herpesvirus (KSHV) maintenance and viral spread in endothelial cells.
Application : WB
Reactivity : Human
Chang CF et al. Cell Death Dis 2015; 6 : e1716 PHRF1 promotes genome integrity by modulating non-homologous end-joining.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169