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NBS1 antibody

Orthogonal
Anti-NBS1 antibody used in Western Blot (WB). GTX30125
Anti-NBS1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30125
Anti-NBS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX30125
Anti-NBS1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30125
Anti-NBS1 antibody used in Western Blot (WB). GTX30125

Cat. No. GTX30125

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS, IP, ChIP assay

Reactivity

 Human, Mouse, Hamster, Primate
Package
50 μl ($449)
See all NBS1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:50 - 1:200
IHC-P 1:100 - 1:200
FACS Assay dependent
IP 3 ul
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

85 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Serum

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Antigen Species

Human

Immunogen

Partial length human NBS1 protein [Swiss-Prot# O60934].

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_386040

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nibrin , AT-V1 , AT-V2 , ATV , NBS , NBS1 , P95

Cellular Localization

Nucleus

Background

Mutations in this gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-NBS1 antibody used in Western Blot (WB). GTX30125

GTX30125 WB Image

WB analysis of HeLa cell lysate using GTX30125 NBS1 antibody.

Anti-NBS1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30125

GTX30125 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX30125 NBS1 antibody.

Anti-NBS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX30125

GTX30125 IHC-P Image

IHC-P analysis of human testis tissue using GTX30125 NBS1 antibody.
Dilution : 1:200
Antigen retrieval : Heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0)

Anti-NBS1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30125

GTX30125 ICC/IF Image

Orthogonal

The data was published in the journal PLoS One in 2015. PMID: 26683461

Anti-NBS1 antibody used in Western Blot (WB). GTX30125

GTX30125 WB Image

The data was published in the journal PLoS One in 2015. PMID: 26683461

REFERENCE

Application Reference

Ge C et al. PLoS One 2015; 10 (12) : e0144957 The UBC Domain Is Required for BRUCE to Promote BRIT1/MCPH1 Function in DSB Signaling and Repair Post Formation of BRUCE-USP8-BRIT1 Complex.
Ge C et al. Proc Natl Acad Sci U S A 2015; 112 (11) : E1210-9 BRUCE regulates DNA double-strand break response by promoting USP8 deubiquitination of BRIT1.
Franchitto A et al. J Cell Biol 2002; 157 (1) : 19-30 Bloom's syndrome protein is required for correct relocalization of RAD50/MRE11/NBS1 complex after replication fork arrest.
Application : ICC/IF
Reactivity : Human
Harfst E et al. Mol Immunol 2000; 37 (15) : 915-29 Normal V(D)J recombination in cells from patients with Nijmegen breakage syndrome.

Clone Reference

Siwicki JK et al. Exp Cell Res 2003; 287 (1):178-89 Telomere maintenance and cell cycle regulation in spontaneously immortalized T-cell lines from Nijmegen breakage syndrome patients.
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SDS
Sodium Azide.pdf
Package List Price ($)
$ 449