GeneTex
United States (US)

NBS1 antibody

GTX30125_180_ICC-IF_18121410_756.jpg
GTX30125_1339_WB_18121410_401.jpg
GTX30125_537_IHC-P_18121410_878.jpg

Cat No. GTX30125

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, FACS, IP, ChIP assay
Reactivity Human, Mouse, Hamster, Primate
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000
ICC/IF 1:50 - 1:200
IHC-P 1:100 - 1:200
FACS Assay dependent
IP 3 ul
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

85 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

Serum, 0.02% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

Batch dependent(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Partial length human NBS1 protein [Swiss-Prot# O60934].

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

AT-V1 antibody , AT-V2 antibody , ATV antibody , NBS antibody , NBS1 antibody , P95 antibody

Cellular Localization

Nucleus (By similarity),Telomere (By similarity)

Background

Mutations in this gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX30125_180_ICC-IF_18121410_756.jpg

GTX30125 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX30125 NBS1 antibody.

GTX30125_1339_WB_18121410_401.jpg

GTX30125 WB Image

WB analysis of HeLa cell lysate using GTX30125 NBS1 antibody.

GTX30125_537_IHC-P_18121410_878.jpg

GTX30125 IHC-P Image

IHC-P analysis of human testis tissue using GTX30125 NBS1 antibody.
Dilution : 1:200
Antigen retrieval : Heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0)

REFERENCE