GeneTex

  • Country / Location Selection

United States (US)

NCALD antibody

Anti-NCALD antibody used in IHC (Paraffin sections) (IHC-P). GTX121264
Anti-NCALD antibody used in IHC (Paraffin sections) (IHC-P). GTX121264
Anti-NCALD antibody used in Western Blot (WB). GTX121264

Cat. No. GTX121264

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all NCALD products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Positive Control

NT2D1 , PC-3 , U87-MG , SK-N-SH

Predict Reactivity

Drosophila, Bovine, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NCALD. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11171613

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

neurocalcin delta

Background

This gene encodes a member of the neuronal calcium sensor (NCS) family of calcium-binding proteins. The protein contains an N-terminal myristoylation signal and four EF-hand calcium binding loops. The protein is cytosolic at resting calcium levels; however, elevated intracellular calcium levels induce a conformational change that exposes the myristoyl group, resulting in protein association with membranes and partial co-localization with the perinuclear trans-golgi network. The protein is thought to be a regulator of G protein-coupled receptor signal transduction. Several alternatively spliced variants of this gene have been determined, all of which encode the same protein; additional variants may exist but their biological validity has not been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-NCALD antibody used in IHC (Paraffin sections) (IHC-P). GTX121264

GTX121264 IHC-P Image

Neurocalcin delta antibody detects Neurocalcin delta protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Neurocalcin delta antibody (GTX121264) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NCALD antibody used in IHC (Paraffin sections) (IHC-P). GTX121264

GTX121264 IHC-P Image

Neurocalcin delta antibody detects Neurocalcin delta protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Neurocalcin delta antibody (GTX121264) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NCALD antibody used in Western Blot (WB). GTX121264

GTX121264 WB Image

Sample (30 μg of whole cell lysate)
A: NT2D1
B: PC-3
C: U87-MG
D: SK-N-SH
12% SDS PAGE
GTX121264 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

There are currently no references for NCALD antibody (GTX121264). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for NCALD antibody (GTX121264). Be the first to share your experience with this product.
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169