NCAM antibody [GT135]

NCAM antibody [GT135] detects NCAM protein by immunohistochemical analysis.
Sample: Paraffin-embedded human hippocampus.
NCAM stained by NCAM antibody [GT135] (GTX634792) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

NCAM antibody [GT135] detects NCAM protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse eye.
Green: NCAM stained by NCAM antibody [GT135] (GTX634792) diluted at 1:250.
Red: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody (GTX130245) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

NCAM antibody [GT135] detects NCAM protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hippocampus.
NCAM stained by NCAM antibody [GT135] (GTX634792) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

NCAM antibody [GT135] detects NCAM protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
Green: NCAM stained by NCAM antibody [GT135] (GTX634792) diluted at 1:250.
Red: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody (GTX130245) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with NCAM antibody [GT135] (GTX634792) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NCAM antibody [GT135] (GTX634792) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

NCAM antibody [GT135] detects NCAM protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NCAM stained by NCAM antibody [GT135] (GTX634792) diluted at 1:500.
Red: NeuN, stained by NeuN antibody (GTX132974) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

NCAM antibody [GT135] detects NCAM protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
NCAM stained by NCAM antibody [GT135] (GTX634792) diluted at 1:400.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min