GeneTex
United States (US)

NCAM antibody [MEM-188]

GTX28233_NCAM-antibody-MEM-188__GTX28233-1_19012723_822.jpeg
NCAM-antibody-MEM-188__GTX28233-1_18121411_589.gif
NCAM-antibody-MEM-188__GTX28233-2_18121411_585.gif

Cat No. GTX28233

Host Mouse
Clonality Monoclonal
Clone Name MEM-188
Isotype IgG2a
Application WB, FACS, IP
Reactivity Human
APPLICATION

Application Note

FACS: Use at an assay dependant concentration. IP: Use at an assay dependant concentration. WB: Use at an assay dependant concentration. Use under non-reducing conditions. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

95 kDa. ( Note )

Specificity/Sensitivity

CD56 antigen (human). This antibody reacts with 180 kDa isoform characteristic for leucocytes. Reactivity with other NCAM isoforms has not been tested.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline pH 7.4 containing 15mM sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Tissue / cell preparation (Human). (KG-1 acute myelogenous leukaemia cell line).

Purification

>95% by SDS-PAGE

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CD56, 116930, NCAM, MSK39, P13591, 4684, NCAM1

Cellular Localization

Type I membrane protein

Background

NCAM, as a member of the immunoglobulin superfamily of adhesion molecules is characterized by several immunoglobulin (Ig)-like domains. The extracellular part of NCAM consists of five of these Ig domains and two fibronectin type III homology regions. NCAM is encoded by a single copy gene composed of 26 exons. However, at least 20-30 distinct isoforms can be generated by alternative splicing and by posttranslational modifications, such as sialylation. During sialylation, polysialic acid (PSA) carbohydrates are attached to the extracellular part of NCAM. Through its extracellular region, NCAM mediates homophilic interactions. In addition, NCAM can also undergo heterophilic interactions by binding extracellular matrix components, such as laminin, or other cell adhesion molecules, such as integrins.

Database

Research Area

DATA IMAGES
GTX28233_NCAM-antibody-MEM-188__GTX28233-1_19012723_822.jpeg

GTX28233 FACS Image

Surface staining of human peripheral blood cells with anti-CD56 (MEM-188): FITC (GTX75715).

NCAM-antibody-MEM-188__GTX28233-1_18121411_589.gif

GTX28233 FACS Image

Staining of human peripheral blood lymphocytes with Mouse anti Human NCAM (GTX28233)

NCAM-antibody-MEM-188__GTX28233-2_18121411_585.gif

GTX28233 FACS Image

Staining of human peripheral blood lymphocytes with Mouse anti Human NCAM:Biotin (GTX75714)

Package List Price ($)
$ 319