NCAM antibody [MY31] (PE-Cy7)

Cat. No. GTX01477-10

Host	Mouse
Clonality	Monoclonal
Clone Name	MY31
Isotype	IgG1
Application	FACS
Reactivity	Human, Chimpanzee, Cynomolgus monkey, Rhesus Monkey
Package	100 test ($269)

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View product citations for antibody GTX01477-10 on CiteAb

PRODUCT

Summary

The MY31 antibody reacts with human CD56, also known as the neural cell adhesion molecule (NCAM), a glycoprotein which is a member of the immunoglobulin superfamily. The 140 kDa isoform of CD56 is expressed on human NK cells and NK-T cells, with increased expression levels on activated NK lymphocytes. The CD56 antigen is also expressed by neurons and is reported to play a role in nervous system development and neural cell-to-cell adhesion. Clone MY31 also reacts with a subset of CD14+ monocytes in non-human primates, and is reported to be cross-reactive with Chimpanzee, Cynomolgus and Rhesus.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
FACS	0.5 μg (5 μl) for 10⁵-10⁸ cells in 100 μl sample per test

Not tested in other applications.

Calculated MW

95 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM NaH₂PO₄, 150mM NaCl, 0.1% Gelatin

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Phycoerythrin-Cyanine7 (PE-Cy7)

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

neural cell adhesion molecule 1 , CD56 , MSK39 , NCAM , NCAM1

Cellular Localization

Cell membrane,Secreted

Background

This gene encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. The encoded protein has been shown to be involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2011]

Database

Gene ID: 4684 NCAM1
UniProt: P13591 NCAM1

Research Area

DATA IMAGES

GTX01477-10 FACS Image

FACS analysis of human peripheral blood lymphocytes using GTX01477-10 NCAM antibody [MY31] (PE-Cy7).
Right panel : co-stained with NCAM antibody [MY31] (PE-Cy7) and Human CD3 antibody (APC)
Left panel : co-stained with isotype control and Human CD3 antibody (APC)
antibody amount : 0.5 μg (5 μl)