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NCAM antibody [OTI1G4]

Overexpression
Anti-NCAM antibody [OTI1G4] used in Western Blot (WB). GTX04078
Anti-NCAM antibody [OTI1G4] used in Western Blot (WB). GTX04078
Anti-NCAM antibody [OTI1G4] used in IHC (Paraffin sections) (IHC-P). GTX04078

Cat. No. GTX04078

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 OTI1G4

Isotype

 IgG1

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat, Dog, Monkey
Package
100 μl ($459)
See all NCAM products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:200-1:4000
IHC-P 1:150
Not tested in other applications.

Calculated MW

95 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human recombinant protein fragment corresponding to amino acids 20-718 of human NCAM1 (NP_851996) produced in HEK293T cells.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

neural cell adhesion molecule 1 , CD56 , MSK39 , NCAM

Background

This gene encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. The encoded protein has been shown to be involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2011]

Database

Research Area

DATA IMAGES

Anti-NCAM antibody [OTI1G4] used in Western Blot (WB). GTX04078

GTX04078 WB Image

WB analysis of various cell lysates using GTX04078 NCAM antibody.
Loading : 35 μg

Anti-NCAM antibody [OTI1G4] used in Western Blot (WB). GTX04078

GTX04078 WB Image

Overexpression

WB analysis of 293T cells transfected with human NCAM using GTX04078 NCAM antibody [OTI1G4].
Lane 1 : 293T cells transfected with control vector
Lane 2 : 293T cells transfected with human NCAM
Loading : 5 μg

Anti-NCAM antibody [OTI1G4] used in IHC (Paraffin sections) (IHC-P). GTX04078

GTX04078 IHC-P Image

IHC-P analysis of human thyroid carcinoma tissue using GTX04078 NCAM antibody.
Antigen retrieval : Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120ºC for 3min

REFERENCE

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REVIEW

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Package List Price ($)
$ 459