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NCAM antibody

Anti-NCAM antibody used in Western Blot (WB). GTX133217
Anti-NCAM antibody used in IHC (Paraffin sections) (IHC-P). GTX133217
Anti-NCAM antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133217
Anti-NCAM antibody used in IHC (Paraffin sections) (IHC-P). GTX133217

Cat. No. GTX133217

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP, ELISA, PLA

Reactivity

 Mouse, Rat, Chinese Hamster
Package
100 μl ($399),
25 μl ($169)
See all NCAM products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
ELISA Assay dependent
PLA Assay dependent
Not tested in other applications.

Calculated MW

119 kDa. ( Note )

Positive Control

mouse brain , rat brain

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.11 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant protein encompassing a sequence within the Intracellular domain of mouse NCAM. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886874

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

neural cell adhesion molecule 1 , CD56 , E-NCAM , NCAM-1 , Ncam

Cellular Localization

Isoform 1: Cell membrane; Single-pass type I membrane protein , Isoform 2: Cell membrane; Single-pass type I membrane protein , Isoform 3: Cell membrane; Lipid-anchor , GPI-anchor

Background

This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.

Database

Research Area

DATA IMAGES

Anti-NCAM antibody used in Western Blot (WB). GTX133217

GTX133217 WB Image

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NCAM antibody (GTX133217) diluted at 1:1250. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NCAM antibody used in IHC (Paraffin sections) (IHC-P). GTX133217

GTX133217 IHC-P Image

NCAM antibody​ detects NCAM protein at cell membrane in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
NCAM antibody​ (GTX133217) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NCAM antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133217

GTX133217 ICC/IF Image

NCAM antibody detects NCAM protein at cell membrane by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NCAM protein stained by NCAM antibody (GTX133217) diluted at 1:1000.
Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-NCAM antibody used in IHC (Paraffin sections) (IHC-P). GTX133217

GTX133217 IHC-P Image

NCAM antibody​ detects NCAM protein at cell membrane in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
NCAM antibody​ (GTX133217) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Amores-Bonet L et al. Int J Mol Sci 2022; 23 (17) Interactions between the Polysialylated Neural Cell Adhesion Molecule and the Transient Receptor Potential Canonical Channels 1, 4, and 5 Induce Entry of Ca(2+) into Neurons.
Application : WB, ICC/IF, IP, ELISA, PLA
Reactivity : Mouse, Chinese Hamster
Wang MJ et al. Med Sci Monit 2019; 25 : 4627-4638 Levetiracetam Protects Against Cognitive Impairment of Subthreshold Convulsant Discharge Model Rats by Activating Protein Kinase C (PKC)-Growth-Associated Protein 43 (GAP-43)-Calmodulin-Dependent Protein Kinase (CaMK) Signal Transduction Pathway.
Application : WB
Reactivity : Rat
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169