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NCAM antibody

Anti-NCAM antibody used in Western Blot (WB). GTX133217
Anti-NCAM antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133217
Anti-NCAM antibody used in IHC (Paraffin sections) (IHC-P). GTX133217
Anti-NCAM antibody used in IHC (Paraffin sections) (IHC-P). GTX133217

Cat No. GTX133217

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Mouse, Rat
Package
100 μl ($379),
25 μl ($169)
See all NCAM products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

119 kDa. ( Note )

Positive Control

mouse brain , rat brain

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.11 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of mouse NCAM. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886874

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

neural cell adhesion molecule 1 , CD56 , E-NCAM , NCAM-1 , Ncam

Cellular Localization

Isoform 1: Cell membrane; Single-pass type I membrane protein , Isoform 2: Cell membrane; Single-pass type I membrane protein , Isoform 3: Cell membrane; Lipid-anchor , GPI-anchor

Background

This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.

Database

Research Area

DATA IMAGES

Anti-NCAM antibody used in Western Blot (WB). GTX133217

GTX133217 WB Image

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NCAM antibody (GTX133217) diluted at 1:1250. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NCAM antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133217

GTX133217 ICC/IF Image

NCAM antibody detects NCAM protein at cell membrane by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NCAM protein stained by NCAM antibody (GTX133217) diluted at 1:1000.
Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-NCAM antibody used in IHC (Paraffin sections) (IHC-P). GTX133217

GTX133217 IHC-P Image

NCAM antibody​ detects NCAM protein at cell membrane in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
NCAM antibody​ (GTX133217) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NCAM antibody used in IHC (Paraffin sections) (IHC-P). GTX133217

GTX133217 IHC-P Image

NCAM antibody​ detects NCAM protein at cell membrane in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
NCAM antibody​ (GTX133217) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Wang MJ et al. Med Sci Monit 2019; 25 : 4627-4638 Levetiracetam Protects Against Cognitive Impairment of Subthreshold Convulsant Discharge Model Rats by Activating Protein Kinase C (PKC)-Growth-Associated Protein 43 (GAP-43)-Calmodulin-Dependent Protein Kinase (CaMK) Signal Transduction Pathway.
Application : WB
Reactivity : Rat
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 379
$ 169