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NCAM antibody

Anti-NCAM antibody used in Western Blot (WB). GTX64346
Anti-NCAM antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64346
Anti-NCAM antibody used in IHC (Paraffin sections) (IHC-P). GTX64346
Anti-NCAM antibody used in IHC (Paraffin sections) (IHC-P). GTX64346

Cat. No. GTX64346

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Mouse, Rat
Package
100 μl ($399)
See all NCAM products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

95 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 759-858 of human NCAM1 (NP_851996.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

neural cell adhesion molecule 1 , CD56 , MSK39 , NCAM

Background

This gene encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. The encoded protein has been shown to be involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2011]

Database

Research Area

DATA IMAGES

Anti-NCAM antibody used in Western Blot (WB). GTX64346

GTX64346 WB Image

WB analysis of various sample lysates using GTX64346 NCAM antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-NCAM antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64346

GTX64346 ICC/IF Image

ICC/IF analysis of C6 cells using GTX64346 NCAM antibody.
Blue : DAPI
Dilution : 1:100

Anti-NCAM antibody used in IHC (Paraffin sections) (IHC-P). GTX64346

GTX64346 IHC-P Image

IHC-P analysis of rat brain tissue using GTX64346 NCAM antibody.
Dilution : 1:100

Anti-NCAM antibody used in IHC (Paraffin sections) (IHC-P). GTX64346

GTX64346 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX64346 NCAM antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399