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NCAM2 antibody, C-term

KO/KD Orthogonal
Anti-NCAM2 antibody, C-term used in IHC (Frozen sections) (IHC-Fr). GTX89311
Anti-NCAM2 antibody, C-term used in IHC (Frozen sections) (IHC-Fr). GTX89311
Anti-NCAM2 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89311

Cat. No. GTX89311

Reference 3

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all NCAM2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr 0.5-3.75μg/ml

Note :

IHC-Fr
Frozen section of Olfactory bulb from transcardially PFA-perfused Mouse shows strong staining of axon bundles and moderate peripheral staining outside the glomeruli.
Not tested in other applications.

Calculated MW

93 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89311-PEP).

Predict Reactivity

Rat, Bovine, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-KVSNDIIQSKEDDSK, from the C Terminus of the protein sequence according to NP_004531.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

RRID

AB_10721561

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

neural cell adhesion molecule 2 , NCAM21

Cellular Localization

Cell membrane; Single-pass type I membrane protein

Background

The protein encoded by this gene belongs to the immunoglobulin superfamily. It is a type I membrane protein and may function in selective fasciculation and zone-to-zone projection of the primary olfactory axons. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-NCAM2 antibody, C-term used in IHC (Frozen sections) (IHC-Fr). GTX89311

GTX89311 IHC-Fr Image

IHC-Fr analysis of PFA-perfused mouse Olfactory bub using GTX89311 NCAM2 antibody, C-term.
Antigen retrieval : methanol (-20C, 10min) followed by 1% SDS(10min)
Dilution : 0.5μg/ml

Anti-NCAM2 antibody, C-term used in IHC (Frozen sections) (IHC-Fr). GTX89311

GTX89311 IHC-Fr Image

IHC-Fr analysis of PFA-perfused mouse Olfactory bulb using GTX89311 NCAM2 antibody, C-term.
Red : NCAM2
Blue : VGLUT2
Green : NQO1
Antigen retrieval : citrate buffer pH 6 at 95ºC for 10min
Dilution : 0.5μg/ml

Anti-NCAM2 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89311

GTX89311 IHC-P Image

IHC-P analysis of human cortex using GTX89311 NCAM2 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75μg/ml

REFERENCE

Application Reference

Keable R et al. Cell Mol Life Sci 2022; 79 (11) : 555 The BACE1-generated C-terminal fragment of the neural cell adhesion molecule 2 (NCAM2) promotes BACE1 targeting to Rab11-positive endosomes.
Application : WB, ICC/IF
Reactivity : Human, Mouse
Kim W et al. J Biol Chem 2021; : 100372 Spatiotemporal processing of neural cell adhesion molecules 1 and 2 by BACE1 in vivo.
Application : WB
Reactivity : Mouse
Sheng L et al. Cereb Cortex 2018; (Epub) Neural Cell Adhesion Molecule 2 (NCAM2)-Induced c-Src-Dependent Propagation of Submembrane Ca2+ Spikes Along Dendrites Inhibits Synapse Maturation.
Application : ICC/IF
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399