GeneTex

  • Country / Location Selection

United States (US)

NCE2 antibody [C1C3]

Anti-NCE2 antibody [C1C3] used in Western Blot (WB). GTX107726

Cat. No. GTX107726

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all NCE2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

UBE2F transfected 293T cell

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.54 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human NCE2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952484

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ubiquitin conjugating enzyme E2 F (putative) , NCE2

Background

Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5.

Database

Research Area

DATA IMAGES

Anti-NCE2 antibody [C1C3] used in Western Blot (WB). GTX107726

GTX107726 WB Image

Western Blot analysis of UBE2F expression in transfected 293T cell line by UBE2F polyclonal antibody.
A: Non-transfected lysate.
B: UBE2F transfected lysate.
12% SDS PAGE
GTX107726 diluted at 1:500

REFERENCE

There are currently no references for NCE2 antibody [C1C3] (GTX107726). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for NCE2 antibody [C1C3] (GTX107726). Be the first to share your experience with this product.
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169