GeneTex
United States (US)

NCF1 antibody

GTX102957_40723_IHC_19011506_872.jpg
GTX102957_40723_WB_19011506_270.jpg
GTX102957_40723_IFA_19011506_254.jpg

Cat No. GTX102957

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

Raji

Predict Reactivity

Mouse, Rat, Rabbit, Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NCF1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

FLJ79451 antibody, NCF1A antibody, NOXO2 antibody, SH3PXD1A antibody, p47phox antibody, NCF1 antibody, NCF-47K antibody, SH3 and PX domain-containing protein 1A antibody, 47 kDa autosomal chronic granulomatous disease protein antibody, NCF-1 antibody, nox organizer 2 antibody, 47 kDa neutrophil oxidase factor antibody, NADPH oxidase organizer 2 antibody, neutrophil NADPH oxidase factor 1 antibody, p47-phox antibody, neutrophil cytosol factor 1 antibody, neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1) antibody, nox-organizing protein 2 antibody, neutrophil cytosolic factor 1 antibody

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX102957_40723_IHC_19011506_872.jpg

GTX102957 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Hela xenograft, using NCF1(GTX102957) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102957_40723_WB_19011506_270.jpg

GTX102957 WB Image

Sample (30 ug of whole cell lysate)
A: Raji
10% SDS PAGE
GTX102957 diluted at 1:5000

GTX102957_40723_IFA_19011506_254.jpg

GTX102957 ICC/IF Image

NCF1 antibody detects NCF1 protein at cytoplasm by immunofluorescent analysis.
Sample: Raji cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NCF1 protein stained by NCF1 antibody (GTX102957) diluted at 1:500.
Blue: Hoechst 33342 staining.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169