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NCF1 (phospho Ser359) antibody

Anti-NCF1 (phospho Ser359) antibody used in Western Blot (WB). GTX55429

Cat. No. GTX55429

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399)
See all NCF1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of Serine 359 (Q-R-S(p)-K-P) derived from human NCF1.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

neutrophil cytosolic factor 1 , NCF1A , NOXO2 , SH3PXD1A , p47phox

Cellular Localization

Cytoplasm, cytosol,Membrane

Background

The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-NCF1 (phospho Ser359) antibody used in Western Blot (WB). GTX55429

GTX55429 WB Image

WB analysis of extracts from HeLa cells using GTX55429 NCF1 (phospho Ser359) antibody.
Left : Primary antibody pre-incubated with the antigen specific peptide
Right : Primary antibody

REFERENCE

Application Reference

Castaldo M et al. Oxid Med Cell Longev 2019; 2019 : 2051235 NOX2-Dependent Reactive Oxygen Species Regulate Formyl-Peptide Receptor 1-Mediated TrkA Transactivation in SH-SY5Y Cells.
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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399