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NCF1 (phospho Ser370) antibody

Anti-NCF1 (phospho Ser370) antibody used in Western Blot (WB). GTX32294
Anti-NCF1 (phospho Ser370) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32294

Cat. No. GTX32294

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all NCF1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium Phosphate, 0.87% NaCl, 30% Glycerol

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of NCF1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

neutrophil cytosolic factor 1 , NCF1A , NOXO2 , SH3PXD1A , p47phox

Cellular Localization

Cytoplasm, cytosol,Membrane

Background

The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-NCF1 (phospho Ser370) antibody used in Western Blot (WB). GTX32294

GTX32294 WB Image

WB analysis of serum starvation-treated HeLa (A), THP1 (B), serum starvation-treated Raw264.7 (C), serum starvation-treated H9C2 (D) whole cell lysates using GTX32294 NCF1 (phospho Ser370) antibody.

Anti-NCF1 (phospho Ser370) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32294

GTX32294 ICC/IF Image

ICC/IF analysis of formalin-fixed THP1 cells using GTX32294 NCF1 (phospho Ser370) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399