GeneTex
United States (US)

NCF1 (phospho Ser370) antibody

GTX32294_20190131_WB_19013102_791.jpg
GTX32294_20190131_ICCIF_19013102_977.jpg

Cat No. GTX32294

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
Not tested in other applications.

Calculated MW

45 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of NCF1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Neutrophil Cytosolic Factor 1,Ncf1A,Noxo2,Sh3Pxd1A,P47Phox,Ncf1

Cellular Localization

Cytoplasm,Cytosol,Membrane

Background

The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX32294_20190131_WB_19013102_791.jpg

GTX32294 WB Image

WB analysis of serum starvation-treated HeLa (A), THP1 (B), serum starvation-treated Raw264.7 (C), serum starvation-treated H9C2 (D) whole cell lysates using GTX32294 NCF1 (phospho Ser370) antibody.

GTX32294_20190131_ICCIF_19013102_977.jpg

GTX32294 ICC/IF Image

ICC/IF analysis of formalin-fixed THP1 cells using GTX32294 NCF1 (phospho Ser370) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

Package List Price ($)
$ 319