APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.3-1μg/ml |
| 2-4μg/ml |
|
Note :
IHC-P
Human Tonsil shows staining of the cytoplasm in some cells.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89030-PEP).
Product Note
This antibody is expected to recognise both reported isoforms (NP_000622.2 and NP_038202.1).
Predict Reactivity
Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-KDFPEEDDPTN, from the internal region of the protein sequence according to NP_000622.2; NP_038202.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
neutrophil cytosolic factor 4 , CGD3 , NCF , P40PHOX , SH3PXD4
Cellular Localization
Cytoplasm, cytosol , Endosome membrane , Membrane
Background
The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX89030 WB Image
WB analysis of Daudi cell lysate using GTX89030 NCF4 antibody, Internal.
Dilution : 0.5μg/ml
Loading : 35μg protein in RIPA buffer
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GTX89030 IHC-P Image
IHC-P analysis of human tonsil using GTX89030 NCF4 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 2.5μg/ml
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GTX89030 WB Image
WB analysis of HEK293 overexpressing NCF4 (mock transfection in first lane) using GTX89030 NCF4 antibody, Internal.
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REFERENCE
There are currently no references for NCF4 antibody, Internal (GTX89030). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for NCF4 antibody, Internal (GTX89030). Be the first to share your experience with this product.
