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NCK1 antibody [5B7]

Anti-NCK1 antibody [5B7] used in Western Blot (WB). GTX60593
Anti-NCK1 antibody [5B7] used in Flow cytometry (FACS). GTX60593
Anti-NCK1 antibody [5B7] used in IHC (Paraffin sections) (IHC-P). GTX60593
Anti-NCK1 antibody [5B7] used in ELISA (ELISA). GTX60593
Anti-NCK1 antibody [5B7] used in Western Blot (WB). GTX60593

Cat. No. GTX60593

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 5B7

Isotype

 IgG1

Application

 WB, IHC-P, FACS, ELISA

Reactivity

 Human, Monkey
Package
100 μg ($409)
See all NCK1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human NCK1 expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NCK adaptor protein 1 , NCK , NCKalpha , nck-1

Cellular Localization

Cytoplasm,Endoplasmic reticulum,Nucleus

Background

The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. Alternatively spliced transcript variants encoding different isoforms have been found. [provided by RefSeq, Jun 2010]

Database

Research Area

DATA IMAGES

Anti-NCK1 antibody [5B7] used in Western Blot (WB). GTX60593

GTX60593 WB Image

WB analysis of human NCK1 (AA: 203-371) recombinant protein using GTX60593 NCK1 antibody [5B7].

Anti-NCK1 antibody [5B7] used in Flow cytometry (FACS). GTX60593

GTX60593 FACS Image

FACS analysis of Jurkat cells using GTX60593 NCK1 antibody [5B7].
Green : NCK1
Red : negative control

Anti-NCK1 antibody [5B7] used in IHC (Paraffin sections) (IHC-P). GTX60593

GTX60593 IHC-P Image

IHC-P analysis of rectum cancer tissue using GTX60593 NCK1 antibody [5B7].

Anti-NCK1 antibody [5B7] used in ELISA (ELISA). GTX60593

GTX60593 ELISA Image

ELISA analysis of antigen using GTX60593 NCK1 antibody [5B7].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

Anti-NCK1 antibody [5B7] used in Western Blot (WB). GTX60593

GTX60593 WB Image

WB analysis of Jurkat (1), HeLa (2), HEK293 (3), A431 (4), K562 (5), and COS7 (6) cell lysate using GTX60593 NCK1 antibody [5B7].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409