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NCK1 antibody

Anti-NCK1 antibody used in Western Blot (WB). GTX111112
Anti-NCK1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111112
Anti-NCK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX111112

Cat. No. GTX111112

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all NCK1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

K562

Predict Reactivity

Mouse, Rat, Sheep, Bovine, Pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.78 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NCK1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11162042

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NCK adaptor protein 1 , NCK , NCKalpha , nck-1

Cellular Localization

Cytoplasm , Endoplasmic reticulum

Background

The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-NCK1 antibody used in Western Blot (WB). GTX111112

GTX111112 WB Image

Sample (30 ug of whole cell lysate)
A: K562
10% SDS PAGE
GTX111112 diluted at 1:1000

Anti-NCK1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111112

GTX111112 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using NCK1(GTX111112) antibody at 1:500 dilution.

Anti-NCK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX111112

GTX111112 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Hela xenograft, using NCK1(GTX111112) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169