GeneTex
United States (US)

NCK1 antibody

GTX32738_20180530_ICCIF_18121410_771.jpg
GTX32738_20180530_WB_18121410_757.jpg

Cat No. GTX32738

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
Not tested in other applications.

Calculated MW

43 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 58-377 of human NCK1 (NP_006144.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Nck Adaptor Protein 1,Nck,Nckalpha,Nck-1,Nck1

Background

The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. Alternatively spliced transcript variants encoding different isoforms have been found. [provided by RefSeq, Jun 2010]

Database

Research Area

DATA IMAGES
GTX32738_20180530_ICCIF_18121410_771.jpg

GTX32738 ICC/IF Image

ICC/IF analysis of A549 cell using GTX32738 NCK1 antibody.
Blue : DAPI

GTX32738_20180530_WB_18121410_757.jpg

GTX32738 WB Image

WB analysis of mouse brain tissue lysate using GTX32738 NCK1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Package List Price ($)
$ 329