GeneTex
United States (US)

NCKAP1 antibody [C1C2], Internal

GTX105682_39890_20180125_IHC-Fr_M_19011506_217.jpg
GTX105682_39890_20171215_WB_B_19011506_481.jpg
GTX105682_39890_WB_M_brain_19011506_357.jpg
GTX105682_39890_WB_R_brain_19011506_175.jpg

Cat No. GTX105682

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-Fr
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

129 kDa. ( Note )

Positive Control

NCKAP1-transfected 293T , Mouse brain , rat brain

Predict Reactivity

Zebrafish, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NCKAP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

FLJ11291 antibody, HEM2 antibody, KIAA0587 antibody, MGC8981 antibody, NAP1 antibody, NAP125 antibody, NCKAP1 antibody, membrane-associated protein HEM-2 antibody, nck-associated protein 1 antibody, p125Nap1 antibody, NAP 1 antibody

Cellular Localization

Cell membrane; Single-pass membrane protein; Cytoplasmic side (By similarity) , Cell projection , lamellipodium membrane; Single-pass membrane protein; Cytoplasmic side (By similarity)

Background

Is part of lamellipodial complex that controls Rac-dependent actin remodeling.

Database

Research Area

DATA IMAGES
GTX105682_39890_20180125_IHC-Fr_M_19011506_217.jpg

GTX105682 IHC-Fr Image

NCKAP1 antibody [C1C2], Internal detects NCKAP1 protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse cerebellum.
Green: NCKAP1 stained by NCKAP1 antibody [C1C2], Internal (GTX105682) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX105682_39890_20171215_WB_B_19011506_481.jpg

GTX105682 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with NCKAP1 antibody [C1C2], Internal (GTX105682) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX105682_39890_WB_M_brain_19011506_357.jpg

GTX105682 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
5% SDS PAGE
GTX105682 diluted at 1:1000

GTX105682_39890_WB_R_brain_19011506_175.jpg

GTX105682 WB Image

NCKAP1 antibody [C1C2], Internal detects NCKAP1 protein by Western blot analysis.
A. 50 µg rat brain lysate/extract
5 % SDS-PAGE
NCKAP1 antibody [C1C2], Internal (GTX105682) dilution: 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169