APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.5-1 μg/mL |
| 20 μg/mL |
| 1 μg/mL |
| Assay dependent |
Not tested in other applications.
Calculated MW
Predict Reactivity
Dog, Monkey, Gorilla, Horse(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide corresponding to 13 amino acids near the center of human NCOA4. The immunogen is located within 420-470 amino acids of NCOA4.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
nuclear receptor coactivator 4 , ARA70 , ELE1 , PTC3 , RFG
Background
This gene encodes an androgen receptor coactivator. The encoded protein interacts with the androgen receptor in a ligand-dependent manner to enhance its transcriptional activity. Chromosomal translocations between this gene and the ret tyrosine kinase gene, also located on chromosome 10, have been associated with papillary thyroid carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes are present on chromosomes 4, 5, 10, and 14. [provided by RefSeq, Feb 2009]
Database
Research Area
DATA IMAGES
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GTX04250 IHC-P Image
IHC-P analysis of mouse lung tissue using GTX04250 NCOA4 antibody.
Antigen retrieval : Heat mediated citrate buffer (pH 6)
Dilution : 1 μg/mL
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GTX04250 WB Image
WB analysis of various tissue lysates using GTX04250 NCOA4 antibody.
Loading : 15 μg
Dilution : 1 μg/mL
Lane 1: Rat lung
Lane 2 : Rat thymus
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GTX04250 IHC-P Image
IHC-P analysis of human lung tissue using GTX04250 NCOA4 antibody.
Antigen retrieval : Heat mediated citrate buffer (pH 6)
Dilution : 1 μg/mL
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GTX04250 WB Image
WB analysis of cell lysates using GTX04250 NCOA4 antibody.
Loading : 15 μg
Dilution : 0.5 μg/mL
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GTX04250 IHC-P Image
IHC-P analysis of rat lung tissue using GTX04250 NCOA4 antibody.
Antigen retrieval : Heat mediated citrate buffer (pH 6)
Dilution : 1 μg/mL
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GTX04250 ICC/IF Image
ICC/IF analysis of 4% PFA-fixed Daudi cells using GTX04250 NCOA4 antibody.
Green : Primary antibody
Blue : DAPI
Dilution : 20 μg/mL
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GTX04250 WB Image
WB analysis of various tissue lysates using GTX04250 NCOA4 antibody.
Loading : 15 μg
Dilution : 1 μg/mL
Lane 1: Mouse kidney
Lane 2 : Mouse lung
Lane 3 : Mouse thymus
Lane 4 : Mouse pancreas
Lane 5 : Mouse stomach
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REFERENCE
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REVIEW
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