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NCOA4 antibody

KO/KD
Anti-NCOA4 antibody used in Western Blot (WB). GTX32739
Anti-NCOA4 antibody used in IHC (Paraffin sections) (IHC-P). GTX32739
Anti-NCOA4 antibody used in Western Blot (WB). GTX32739

Cat. No. GTX32739

Reference 2
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all NCOA4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:100
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 495-614 of human NCOA4 (NP_005428.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887680

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nuclear receptor coactivator 4 , ARA70 , ELE1 , PTC3 , RFG

Background

This gene encodes an androgen receptor coactivator. The encoded protein interacts with the androgen receptor in a ligand-dependent manner to enhance its transcriptional activity. Chromosomal translocations between this gene and the ret tyrosine kinase gene, also located on chromosome 10, have been associated with papillary thyroid carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes are present on chromosomes 4, 5, 10, and 14. [provided by RefSeq, Feb 2009]

Database

Research Area

DATA IMAGES

Anti-NCOA4 antibody used in Western Blot (WB). GTX32739

GTX32739 WB Image

WB analysis of various sample lysates using GTX32739 NCOA4 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-NCOA4 antibody used in IHC (Paraffin sections) (IHC-P). GTX32739

GTX32739 IHC-P Image

IHC-P analysis of human lung cancer tissue using GTX32739 NCOA4 antibody.
Dilution : 1:100

Anti-NCOA4 antibody used in Western Blot (WB). GTX32739

GTX32739 WB Image

KO/KD

WB analysis of normal (control) and knockout (KO) HeLa cell lysate using GTX32739 NCOA4 antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

Application Reference

LT Tsai et al. Antioxidants (Basel) 2023; 12 (11) : 1931 Inhibition of Indoxyl Sulfate-Induced Reactive Oxygen Species-Related Ferroptosis Alleviates Renal Cell Injury In Vitro and Chronic Kidney Disease Progression In Vivo
Application : WB
Homma T et al. Exp Cell Res 2019; : 111592 Edaravone, a free radical scavenger, protects against ferroptotic cell death in vitro.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

NCOA4 antibody Cat. No. GTX32739

Rating
( Average 3 based on 1 users reviews)
Application
Western Blot(WB)
( Average 3 based on 1 users reviews)
Anti-NCOA4 antibody used in Western Blot (WB). GTX32739
Date : Anonymous submitted on 22-Apr-2019
Score :
Application Tested : WB
Sample Species : Ms
Sample : Hepa 1-6 cells
Amount used : 20 μg
Blocking : 5% Skim milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399