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NCOA62 antibody [N3C3]

Anti-NCOA62 antibody [N3C3] used in Western Blot (WB). GTX109978
Anti-NCOA62 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109978
Anti-NCOA62 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX109978
Anti-NCOA62 antibody [N3C3] used in Western Blot (WB). GTX109978

Cat. No. GTX109978

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all NCOA62 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Positive Control

Mouse liver , Raji

Predict Reactivity

Rat, Zebrafish, Bovine, Xenopus tropicalis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.85 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NCOA62. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037492

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SNW domain containing 1 , Bx42 , FUN20 , NCOA-62 , PRPF45 , Prp45 , SKIIP , SKIP , SKIP1

Cellular Localization

Nucleus

Background

This gene, a member of the SNW gene family, encodes a coactivator that enhances transcription from some Pol II promoters. This coactivator can bind to the ligand-binding domain of the vitamin D receptor and to retinoid receptors to enhance vitamin D-, retinoic acid-, estrogen-, and glucocorticoid-mediated gene expression. It can also function as a splicing factor by interacting with poly(A)-binding protein 2 to directly control the expression of muscle-specific genes at the transcriptional level. Finally, the protein may be involved in oncogenesis since it interacts with a region of SKI oncoproteins that is required for transforming activity. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-NCOA62 antibody [N3C3] used in Western Blot (WB). GTX109978

GTX109978 WB Image

Sample (30 ug of whole cell lysate)
A: Raji
7.5% SDS PAGE
GTX109978 diluted at 1:10000

Anti-NCOA62 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109978

GTX109978 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NCOA62(GTX109978) antibody at 1:500 dilution.

Anti-NCOA62 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX109978

GTX109978 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft , using NCOA62 (GTX109978) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NCOA62 antibody [N3C3] used in Western Blot (WB). GTX109978

GTX109978 WB Image

Sample (50 ug of whole cell lysate)
A: mouse liver
7.5% SDS PAGE
GTX109978 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169