APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1 μg/ml |
| 1:100-1:500 |
| 1:200-1:1000 |
| 3-5 μg/10⁶ cells |
| Assay dependent |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
PROPERTIES
Form
Liquid
Buffer
PBS, 1mg/ml BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.7 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Mouse
Immunogen
Synthetic peptide corresponding to residues C P(2427) A P L L S A Q Y E T L S D S D D(2443) of mouse N-CoR.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
nuclear receptor co-repressor 1 , 5730405M06Rik , A230020K14Rik , N-CoR , RIP13 , Rxrip13 , mKIAA1047
Background
Steroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand-dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcription activity of the nuclear receptor with which they associate. Silencing mediator of retinoic acid & thyroid hormone receptor (SMRT) and nuclear receptor corepressor (N-CoR) are related transcriptional corepressors which contain two distinct domains capable of interacting with unliganded nuclear receptors to repress their basal transcriptional activity.
Database
Research Area
DATA IMAGES
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GTX23482 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX23482 NCOR1 antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:200 incubated overnight at 4 ºC
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GTX23482 IHC-P Image
IHC-P analysis of human breast carcinoma tissue using GTX23482 NCOR1 antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:200
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GTX23482 ICC/IF Image
ICC/IF analysis of MCF-7 cells using GTX23482 NCOR1 antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:200 incubated overnight at 4 ºC
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GTX23482 IHC-P Image
IHC-P analysis of mouse breast tissue using GTX23482 NCOR1 antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:200
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REFERENCE
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REVIEW
There are currently no reviews for NCOR1 antibody (GTX23482). Be the first to share your experience with this product.
