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NCOR1 antibody

Anti-NCOR1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23482
Anti-NCOR1 antibody used in IHC (Paraffin sections) (IHC-P). GTX23482
Anti-NCOR1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23482
Anti-NCOR1 antibody used in IHC (Paraffin sections) (IHC-P). GTX23482

Cat No. GTX23482

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, FACS, IP, ChIP assay, IHC

Reactivity

Human, Mouse
Package
50 μg ($379)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 μg/ml
ICC/IF 1:100-1:500
IHC-P 1:200-1:1000
FACS 3-5 μg/10⁶ cells
IP Assay dependent
ChIP assay Assay dependent
IHC Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, 1mg/ml BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.7 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Synthetic peptide corresponding to residues C P(2427) A P L L S A Q Y E T L S D S D D(2443) of mouse N-CoR.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

nuclear receptor co-repressor 1 , 5730405M06Rik , A230020K14Rik , N-CoR , RIP13 , Rxrip13 , mKIAA1047

Background

Steroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand-dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcription activity of the nuclear receptor with which they associate. Silencing mediator of retinoic acid & thyroid hormone receptor (SMRT) and nuclear receptor corepressor (N-CoR) are related transcriptional corepressors which contain two distinct domains capable of interacting with unliganded nuclear receptors to repress their basal transcriptional activity.

Database

Research Area

DATA IMAGES

Anti-NCOR1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23482

GTX23482 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX23482 NCOR1 antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:200 incubated overnight at 4 ºC

Anti-NCOR1 antibody used in IHC (Paraffin sections) (IHC-P). GTX23482

GTX23482 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX23482 NCOR1 antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:200

Anti-NCOR1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23482

GTX23482 ICC/IF Image

ICC/IF analysis of MCF-7 cells using GTX23482 NCOR1 antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:200 incubated overnight at 4 ºC

Anti-NCOR1 antibody used in IHC (Paraffin sections) (IHC-P). GTX23482

GTX23482 IHC-P Image

IHC-P analysis of mouse breast tissue using GTX23482 NCOR1 antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:200

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 379