GeneTex
United States (US)

NCS1 antibody

GTX105552_39757_IHC_M_19011506_503.jpg
GTX105552_WB_19011506_774.jpg
GTX105552_39757_IFA_19011506_672.jpg

Cat No. GTX105552

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:100-1:1000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Positive Control

A431 , HepG2

Predict Reactivity

Rat, Bovine, Chicken, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.35mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NCS1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Neuronal Calcium Sensor 1,Flup,Freq,Ncs1

Cellular Localization

Golgi apparatus , Golgi stack membrane; Peripheral membrane protein

Background

This gene is a member of the neuronal calcium sensor gene family, which encode calcium-binding proteins expressed predominantly in neurons. The protein encoded by this gene regulates G protein-coupled receptor phosphorylation in a calcium-dependent manner and can substitute for calmodulin. The protein is associated with secretory granules and modulates synaptic transmission and synaptic plasticity. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX105552_39757_IHC_M_19011506_503.jpg

GTX105552 IHC-P Image

NCS1 antibody detects NCS1 protein at on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
NCS1 antibody (GTX105552) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX105552_WB_19011506_774.jpg

GTX105552 WB Image

Sample(30 μg of whole cell lysate)
A:A431(GTX27909)
B:Hep G2(GTX27900)
12% SDS PAGE
GTX105552 diluted at 1:200

GTX105552_39757_IFA_19011506_672.jpg

GTX105552 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed A431, using NCS1(GTX105552) antibody (Green) at 1:100 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:500.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169