APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Dilution |
1:500 - 1:2000 |
1:50 - 1:200 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS pH7.3, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human NDE1 (NP_060138.1).
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
nudE neurodevelopment protein 1 , HOM-TES-87 , LIS4 , MHAC , NDE , NUDE , NUDE1
Cellular Localization
Cytoplasm, cytoskeleton
Background
This gene encodes a member of the nuclear distribution E (NudE) family of proteins. The encoded protein is localized at the centrosome and interacts with other centrosome components as part of a multiprotein complex that regulates dynein function. This protein plays an essential role in microtubule organization, mitosis and neuronal migration. Mutations in this gene cause lissencephaly 4, a disorder characterized by lissencephaly, severe brain atrophy, microcephaly, and severe cognitive disability. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2012]
Database
Research Area
DATA IMAGES
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GTX33347 WB Image
WB analysis of various sample lysates using GTX33347 NDE1 antibody. Dilution : 1:1000 Loading : 25μg per lane
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GTX33347 IHC-P Image
IHC-P analysis of rat brain tissue using GTX33347 NDE1 antibody. Dilution : 1:100
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GTX33347 IHC-P Image
IHC-P analysis of rat spleen tissue using GTX33347 NDE1 antibody. Dilution : 1:100
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GTX33347 IHC-P Image
IHC-P analysis of human breast cancer tissue using GTX33347 NDE1 antibody. Dilution : 1:100
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REFERENCE
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REVIEW
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