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NDP52 antibody [9E2F2]

Anti-NDP52 antibody [9E2F2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04863
Anti-NDP52 antibody [9E2F2] used in Flow cytometry (FACS). GTX04863
Anti-NDP52 antibody [9E2F2] used in Western Blot (WB). GTX04863

Cat. No. GTX04863

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 9E2F2

Isotype

 IgG2b

Application

 WB, ICC/IF, FACS

Reactivity

 Human, Mouse, Rat
Package
100 μg ($469)
See all NDP52 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.25-0.5 μg/ml
ICC/IF 5 μg/ml
FACS 1-3μg/1x10⁶ cells
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄

Preservative

No preservatives

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

E.coli-derived human NDP52/CALCOCO2 recombinant protein (Position: M1-L446).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CALCOCO2,NDP52,calcium binding and coiledcoil domain 2,calcium binding and coiled-coil domain 2

Cellular Localization

Cytoplasm,Perinuclear region,Golgi apparatus,Cytoskeleton

Background

This gene encodes a coiled-coil domain-containing protein. The encoded protein functions as a receptor for ubiquitin-coated bacteria and plays an important role in innate immunity by mediating macroautophagy. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]

Database

Research Area

DATA IMAGES

Anti-NDP52 antibody [9E2F2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04863

GTX04863 ICC/IF Image

ICC/IF analysis of PFA-fixed U2OS cells using GTX04863 NDP52 antibody [9E2F2].
Orange : Primary antibody
Blue : DAPI
Antigen retrieval : IHC enzyme antigen retrieval reagent for 15 mins
Dilution : 5 μg/mL

Anti-NDP52 antibody [9E2F2] used in Flow cytometry (FACS). GTX04863

GTX04863 FACS Image

FACS analysis of MCF7 cells using GTX04863 NDP52 antibody [9E2F2].
Blue line : Primary antibody
Green line : Isotype control
Red line : Unlabelled sample
Dilution : 1μg/1x10⁶ cells

Anti-NDP52 antibody [9E2F2] used in Western Blot (WB). GTX04863

GTX04863 WB Image

WB analysis of various samples using GTX04863 NDP52 antibody [9E2F2].
Lane 1: Human placenta tissue lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : A549 whole cell lysate
Lane 5 : Rat testis tissue lysate
Lane 6 : Mouse testis tissue lysate
Loading : 30μg
Dilution : 0.5 μg/mL

REFERENCE

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REVIEW

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Package List Price ($)
$ 469