GeneTex

  • Country / Location Selection

United States (US)

NDP52 antibody [GT1813]

KO/KD Orthogonal
Anti-NDP52 antibody [GT1813] used in Western Blot (WB). GTX630397
Anti-NDP52 antibody [GT1813] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630397
Anti-NDP52 antibody [GT1813] used in Western Blot (WB). GTX630397
Anti-NDP52 antibody [GT1813] used in Western Blot (WB). GTX630397
Anti-NDP52 antibody [GT1813] used in Western Blot (WB). GTX630397
Anti-NDP52 antibody [GT1813] used in Western Blot (WB). GTX630397

Cat. No. GTX630397

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT1813

Isotype

 IgG2a

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all NDP52 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

Hep3B , Jurkat , Raji , NCI-H929 , Huh-7 (3 μM Thapsigargin treatment for 12 hr) , Huh-7 (3 μM Thapsigargin treatment for 24 hr) , HepG2 (3 μM Thapsigargin treatment for 12 hr) , HepG2 (3 μM Thapsigargin treatment for 24 hr) , HepG2

Predict Reactivity

Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NDP52. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888208

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

calcium binding and coiled-coil domain 2 , NDP52

Cellular Localization

Cytoplasm , perinuclear region , Golgi apparatus , cytoskeleton

Background

The protein encoded by this gene is a subunit of nuclear domain 10 (ND10) bodies. ND10 bodies are nuclear domains appearing immunohistochemically as ten dots per nucleus. They are believed to be associated with the nuclear matrix on the basis of their resistance to nuclease digestion and salt extraction. ND10 proteins are removed from the nucleus by herpes simplex virus-1 infection and may have a role in viral life cycles. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-NDP52 antibody [GT1813] used in Western Blot (WB). GTX630397

GTX630397 WB Image

Orthogonal

NDP52 antibody detects NDP52 protein by western blot analysis. Un-treated (-) and treated (+, Thapsigargin treatment for 12hrs and 24hrs) Huh-7 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody (GTX630397) diluted by 1:500.
The ACTB was used as internal control (GTX110564, 1:50000) shown at the bottom panel.

Anti-NDP52 antibody [GT1813] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630397

GTX630397 ICC/IF Image

NDP52 antibody [GT1813] detects NDP52 protein at autophagosome by immunofluorescent analysis.
Samples: HeLa cells mock (left) and treated with 50μM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NDP52 protein stained by NDP52 antibody [GT1813] (GTX630397) diluted at 1:1000.
Red: Phalloidin, a F-actin marker.
Anti-NDP52 antibody [GT1813] used in Western Blot (WB). GTX630397

GTX630397 WB Image

KO/KD

Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody [GT1813] (GTX630397) diluted at 1:1000.

Anti-NDP52 antibody [GT1813] used in Western Blot (WB). GTX630397

GTX630397 WB Image

Orthogonal

NDP52 antibody detects NDP52 protein by western blot analysis. Un-treated (-) and treated (+, Thapsigargin treatment for 12hrs and 24hrs) HepG2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody (GTX630397) diluted by 1:500.
The ACTB was used as internal control (GTX110564, 1:50000) shown at the bottom panel.

Anti-NDP52 antibody [GT1813] used in Western Blot (WB). GTX630397

GTX630397 WB Image

NDP52 antibody [GT1813] detects NDP52 protein by western blot analysis.
A. 30 μg Jurkat whole cell lysate/extract
B. 30 μg Raji whole cell lysate/extract
C. 30 μg NCI-H929 whole cell lysate/extract
10 % SDS-PAGE
NDP52 antibody [GT1813] (GTX630397) dilution: 1:1000

Anti-NDP52 antibody [GT1813] used in Western Blot (WB). GTX630397

GTX630397 WB Image

NDP52 antibody [GT1813] detects NDP52 protein by western blot analysis.
A. 30 μg Huh7 whole cell lysate/extract
B. 30 μg Hep3B whole cell lysate/extract
C. 30 μg HepG2 whole cell lysate/extract
10 % SDS-PAGE
NDP52 antibody [GT1813] (GTX630397) dilution: 1:1000

REFERENCE

There are currently no references for NDP52 antibody [GT1813] (GTX630397). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for NDP52 antibody [GT1813] (GTX630397). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations