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NDP52 antibody [GT422]

Anti-NDP52 antibody [GT422] used in Western Blot (WB). GTX630396
Anti-NDP52 antibody [GT422] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630396
Anti-NDP52 antibody [GT422] used in Western Blot (WB). GTX630396
Anti-NDP52 antibody [GT422] used in Immunoprecipitation (IP). GTX630396
Anti-NDP52 antibody [GT422] used in Western Blot (WB). GTX630396
Anti-NDP52 antibody [GT422] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630396
Anti-NDP52 antibody [GT422] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630396
Anti-NDP52 antibody [GT422] used in Western Blot (WB). GTX630396
Anti-NDP52 antibody [GT422] used in Western Blot (WB). GTX630396

Cat No. GTX630396

Host

Mouse

Clonality

Monoclonal

Clone Name

GT422

Isotype

IgG1

Application

WB, ICC/IF, IP

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

Jurkat , Raji , NCI-H929 , Huh-7 , Hep3B , HepG2 , Huh-7 (untreated) , Huh-7 (3 μM Thapsigargin treatment for 12 hr) , Huh-7 (3 μM Thapsigargin treatment for 24 hr) , HepG2 (untreated) , HepG2 (3 μM Thapsigargin treatment for 12 hr)

Predict Reactivity

Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NDP52. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888207

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

calcium binding and coiled-coil domain 2 , NDP52

Cellular Localization

Cytoplasm , perinuclear region , Golgi apparatus , cytoskeleton

Background

The protein encoded by this gene is a subunit of nuclear domain 10 (ND10) bodies. ND10 bodies are nuclear domains appearing immunohistochemically as ten dots per nucleus. They are believed to be associated with the nuclear matrix on the basis of their resistance to nuclease digestion and salt extraction. ND10 proteins are removed from the nucleus by herpes simplex virus-1 infection and may have a role in viral life cycles. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-NDP52 antibody [GT422] used in Western Blot (WB). GTX630396

GTX630396 WB Image

NDP52 antibody detects NDP52 protein by western blot analysis. Un-treated (-) and treated (+, Thapsigargin treatment for 12hrs and 24hrs) Huh-7 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody (GTX630396) diluted by 1:500.
The ACTB was used as internal control (GTX110564, 1:50000) shown at the bottom panel.

Anti-NDP52 antibody [GT422] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630396

GTX630396 ICC/IF Image

NDP52 antibody [GT422] detects NDP52 protein at autophagosome  by immunofluorescent analysis.
Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NDP52 stained by NDP52 antibody [GT422] (GTX630396) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.

Anti-NDP52 antibody [GT422] used in Western Blot (WB). GTX630396

GTX630396 WB Image

NDP52 antibody [GT422] detects NDP52 protein by western blot analysis.
A. 30 μg Jurkat whole cell lysate/extract
B. 30 μg Raji whole cell lysate/extract
C. 30 μg NCI-H929 whole cell lysate/extract
10 % SDS-PAGE
NDP52 antibody [GT422] (GTX630396) dilution: 1:1000

Anti-NDP52 antibody [GT422] used in Immunoprecipitation (IP). GTX630396

GTX630396 IP Image

Immunoprecipitation of NDP52 protein from Jurkat whole cell extracts using 5 μg of NDP52 antibody [GT422] (GTX630396).
Western blot analysis was performed using NDP52 antibody [GT422] (GTX630396).
EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.

Anti-NDP52 antibody [GT422] used in Western Blot (WB). GTX630396

GTX630396 WB Image

NDP52 antibody detects NDP52 protein by western blot analysis. Un-treated (-) and treated (+, Thapsigargin treatment for 12hrs) HepG2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody (GTX630396) diluted by 1:500.
The ACTB was used as internal control (GTX110564, 1:50000) shown at the bottom panel.

Anti-NDP52 antibody [GT422] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630396

GTX630396 ICC/IF Image

NDP52 antibody [GT422] detects NDP52 protein at autophagosome by immunofluorescent analysis.
Samples: HeLa cells mock (left) and treated with 50μM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NDP52 protein stained by NDP52 antibody [GT422] (GTX630396) diluted at 1:1000.
Red: Phalloidin, a F-actin marker.

Anti-NDP52 antibody [GT422] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630396

GTX630396 ICC/IF Image

NDP52 antibody [GT422] detects NDP52 protein at autophagosome by immunofluorescent analysis.
Samples: Hep G2 cells mock (left) and treated with 3 μM Thapsigargin for 16 hrs (right) were fixed in ice-cold MeOH for 10 min and permeabilized with 100% MeOH for 30 sec.
Green: NDP52 protein stained by NDP52 antibody [GT422] (GTX630396) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-NDP52 antibody [GT422] used in Western Blot (WB). GTX630396

GTX630396 WB Image

Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody [GT422] (GTX630396) diluted at 1:1000.

Anti-NDP52 antibody [GT422] used in Western Blot (WB). GTX630396

GTX630396 WB Image

NDP52 antibody [GT422] detects NDP52 protein by western blot analysis.
A. 30 μg Huh7 whole cell lysate/extract
B. 30 μg Hep3B whole cell lysate/extract
C. 30 μg HepG2 whole cell lysate/extract
10 % SDS-PAGE
NDP52 antibody [GT422] (GTX630396) dilution: 1:1000

REFERENCE

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 329
$ 169