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NDP52 antibody

KO/KD Orthogonal
Anti-NDP52 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115378
Anti-NDP52 antibody used in Western Blot (WB). GTX115378
Anti-NDP52 antibody used in Immunoprecipitation (IP). GTX115378
Anti-NDP52 antibody used in Western Blot (WB). GTX115378
Anti-NDP52 antibody used in IHC (Paraffin sections) (IHC-P). GTX115378
Anti-NDP52 antibody used in Western Blot (WB). GTX115378
Anti-NDP52 antibody used in Western Blot (WB). GTX115378
Anti-NDP52 antibody used in Western Blot (WB). GTX115378

Cat. No. GTX115378

Reference 13
4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all NDP52 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

Raji , HepG2 (3 μM Thapsigargin treatment for 12 hr) , HepG2 (3 μM Thapsigargin treatment for 24 hr) , Huh-7 (3 μM Thapsigargin treatment for 12 hr) , Huh-7 (3 μM Thapsigargin treatment for 24 hr)

Predict Reactivity

Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS pH7, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.57 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NDP52. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10620266

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

calcium binding and coiled-coil domain 2 , NDP52

Cellular Localization

Cytoplasm , perinuclear region , Golgi apparatus , cytoskeleton

Background

The protein encoded by this gene is a subunit of nuclear domain 10 (ND10) bodies. ND10 bodies are nuclear domains appearing immunohistochemically as ten dots per nucleus. They are believed to be associated with the nuclear matrix on the basis of their resistance to nuclease digestion and salt extraction. ND10 proteins are removed from the nucleus by herpes simplex virus-1 infection and may have a role in viral life cycles. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-NDP52 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115378

GTX115378 ICC/IF Image

Orthogonal

NDP52 antibody detects NDP52 protein at autophagosome  by immunofluorescent analysis.
Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NDP52 stained by NDP52 antibody (GTX115378) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.

Anti-NDP52 antibody used in Western Blot (WB). GTX115378

GTX115378 WB Image

Orthogonal

Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody (GTX115378) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NDP52 antibody used in Immunoprecipitation (IP). GTX115378

GTX115378 IP Image

Immunoprecipitation of NDP52 protein from Jurkat whole cell extracts using 5 μg of NDP52 antibody (GTX115378).
Western blot analysis was performed using NDP52 antibody (GTX115378).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-NDP52 antibody used in Western Blot (WB). GTX115378

GTX115378 WB Image

KO/KD

Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody (GTX115378) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NDP52 antibody used in IHC (Paraffin sections) (IHC-P). GTX115378

GTX115378 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H441 xenograft, using NDP52(GTX115378) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NDP52 antibody used in Western Blot (WB). GTX115378

GTX115378 WB Image

Orthogonal

NDP52 antibody detects NDP52 protein by western blot analysis. Un-treated (-) and treated (+, Thapsigargin treatment for 12hrs and 24hrs) HepG2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody (GTX115378) diluted by 1:2000.
The ACTB was used as internal control (GTX110564, 1:50000) shown at the bottom panel.
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NDP52 antibody used in Western Blot (WB). GTX115378

GTX115378 WB Image

Sample (30 μg of whole cell lysate)
A: Raji
10% SDS PAGE
GTX115378 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NDP52 antibody used in Western Blot (WB). GTX115378

GTX115378 WB Image

Orthogonal

NDP52 antibody detects NDP52 protein by western blot analysis. Un-treated (-) and treated (+, Thapsigargin treatment for 12hrs and 24hrs) Huh-7 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody (GTX115378) diluted by 1:2000.
The ACTB was used as internal control (GTX110564, 1:50000) shown at the bottom panel.
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Dos Santos ? et al. Nat Commun 2023; 14 (1) : 2855 Autophagy receptor NDP52 alters DNA conformation to modulate RNA polymerase II transcription.
Shiho Fukushima et al. Front Cell Infect Microbiol 2022; 12 : 829682 Recruitment of LC3 by Campylobacter jejuni to Bacterial Invasion Site on Host Cells via the Rac1-Mediated Signaling Pathway.
Application : WB
Reactivity : Human
Po-Yuan Ke et al. Cells 2022; 11 (7) Baicalein Activates Parkin-Dependent Mitophagy through NDP52 and OPTN.
Reactivity : Human
Sabrina Robichaud et al. Autophagy 2021; 17 (11) : 3671-3689 Identification of novel lipid droplet factors that regulate lipophagy and cholesterol efflux in macrophage foam cells.
Application : ICC/IF
Reactivity : Human
Hall BS et al. Autophagy 2021; : 1-19 Inhibition of the SEC61 translocon by mycolactone induces a protective autophagic response controlled by EIF2S1-dependent translation that does not require ULK1 activity.
Application : ICC/IF
Reactivity : Human
Show More +
Submit a Reference

REVIEW

NDP52 antibody Cat. No. GTX115378

Rating
( Average 4.8 based on 4 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 4.7 based on 3 users reviews)
Anti-NDP52 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115378
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Dev Cell in 2019.PMID: 31353311
Anti-NDP52 antibody used in Western Blot (WB). GTX115378
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2017. PMID: 29187741
Anti-NDP52 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115378
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2017. PMID: 29187741
Anti-NDP52 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115378
Date : Anonymous submitted on 03-May-2017
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : HeLa cells
Blocking : 5% BSA+10 mM glycine in PBS, 25°C, 1Hr
Fixation : 4% Paraformaldehyde in PBS
Permeabilization /Antigen retrieval : 0.1% Triton X in PBS
Primary Antibody : 1:500, 4°C, 16Hr
Submit a Review
Application Reference
Dos Santos ? et al. Nat Commun 2023; 14 (1):2855 Autophagy receptor NDP52 alters DNA conformation to modulate RNA polymerase II transcription.
Shiho Fukushima et al. Front Cell Infect Microbiol 2022; 12:829682 Recruitment of LC3 by Campylobacter jejuni to Bacterial Invasion Site on Host Cells via the Rac1-Mediated Signaling Pathway.
Application : WB
Reactivity : Human
Po-Yuan Ke et al. Cells 2022; 11 (7) Baicalein Activates Parkin-Dependent Mitophagy through NDP52 and OPTN.
Reactivity : Human
Sabrina Robichaud et al. Autophagy 2021; 17 (11):3671-3689 Identification of novel lipid droplet factors that regulate lipophagy and cholesterol efflux in macrophage foam cells.
Application : ICC/IF
Reactivity : Human
Hall BS et al. Autophagy 2021;:1-19 Inhibition of the SEC61 translocon by mycolactone induces a protective autophagic response controlled by EIF2S1-dependent translation that does not require ULK1 activity.
Application : ICC/IF
Reactivity : Human
Hung CM et al. Sci Adv 2021; 7 (15) AMPK/ULK1-mediated phosphorylation of Parkin ACT domain mediates an early step in mitophagy.
Application : ICC/IF
Reactivity : Mouse
Ogawa M et al. Commun Biol 2020; 3 (1):25 Streptococcus pneumoniae triggers hierarchical autophagy through reprogramming of LAPosome-like vesicles via NDP52-delocalization.
Wang X et al. Biomed Pharmacother 2020; 127:110125 Mechanisms underlying astrocytic connexin-43 autophagy degradation during cerebral ischemia injury and the effect on neuroinflammation and cell apoptosis.
Application : WB
Reactivity : Mouse
Zachari M et al. Dev Cell 2019; 50 (5):627-643.e5 Selective Autophagy of Mitochondria on a Ubiquitin-Endoplasmic-Reticulum Platform.
Application : ICC/IF
Reactivity : Human
Liao CC et al. Autophagy 2018; 14 (12):2065-2082 Autophagic degradation of SQSTM1 inhibits ovarian cancer motility by decreasing DICER1 and AGO2 to induce MIRLET7A-3P.
Fili N et al. Nat Commun 2017; 8 (1):1871 NDP52 activates nuclear myosin VI to enhance RNA polymerase II transcription.
Application : WB, IP
Reactivity : Human
Chen CH et al. Clin Cancer Res 2017; (Epub) Dual Inhibition of PIK3C3 and FGFR as a New Therapeutic Approach to Treat Bladder Cancer.
Application : WB
Kimura T et al. Acta Neuropathol Commun 2017; 5 (1):67 Age-dependent changes in synaptic plasticity enhance tau oligomerization in the mouse hippocampus.
Application : WB
Reactivity : Mouse
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169