GeneTex
United States (US)

NDP52 antibody

KO/KD Orthogonal
GTX115378_40302_20141230_IFA_18121410_818.jpg
GTX115378_40302_IHC_18121410_313.jpg
GTX115378_40302_20150317_IP_18121410_897.jpg
GTX115378_40302_20141204_WB_Thapsigargin_18121410_928.jpg
GTX115378_40302_20160721_WB_shRNA_watermark_18121410_267.jpg
GTX115378_40302_20141204_WB_Thapsigargin_2_18121410_627.jpg
GTX115378_40302_WB_18121410_249.jpg
GTX115378_40302_IFA_18121410_194.jpg

Cat No. GTX115378

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

Raji , HepG2 , HepG2 whole cell extract (untreated) , HepG2 whole cell extract (3 μM Thapsigargin treatment for 12 hr) , HepG2 whole cell extract (3 μM Thapsigargin treatment for 24 hr) , Huh-7 (untreated) , Huh-7 (3 μM Thapsigargin treatment for 12 hr) , Huh-7 (3 μM Thapsigargin treatment for 24 hr)

Predict Reactivity

Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NDP52. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

MGC17318 antibody, NDP52 antibody, CALCOCO2 antibody, nuclear domain 10 protein NDP52 antibody, nuclear dot protein 52 antibody, antigen nuclear dot 52 kDa protein antibody, nuclear domain 10 protein 52 antibody, calcium-binding and coiled-coil domain-containing protein 2 antibody, calcium binding and coiled-coil domain 2 antibody

Cellular Localization

Cytoplasm , perinuclear region , Golgi apparatus , cytoskeleton

Background

The protein encoded by this gene is a subunit of nuclear domain 10 (ND10) bodies. ND10 bodies are nuclear domains appearing immunohistochemically as ten dots per nucleus. They are believed to be associated with the nuclear matrix on the basis of their resistance to nuclease digestion and salt extraction. ND10 proteins are removed from the nucleus by herpes simplex virus-1 infection and may have a role in viral life cycles. [provided by RefSeq]
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Database

Research Area

DATA IMAGES
GTX115378_40302_20141230_IFA_18121410_818.jpg

GTX115378 ICC/IF Image

NDP52 antibody detects NDP52 protein at autophagosome by immunofluorescent analysis.
Samples: HeLa cells mock (left) and treated with 50μM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NDP52 protein stained by NDP52 antibody (GTX115378) diluted at 1:1000.
Red: Phalloidin, a F-actin marker.

GTX115378_40302_IHC_18121410_313.jpg

GTX115378 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H441 xenograft, using NDP52(GTX115378) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX115378_40302_20150317_IP_18121410_897.jpg

GTX115378 IP Image

Immunoprecipitation of NDP52 protein from Jurkat whole cell extracts using 5 μg of NDP52 antibody (GTX115378).
Western blot analysis was performed using NDP52 antibody (GTX115378).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX115378_40302_20141204_WB_Thapsigargin_18121410_928.jpg

GTX115378 WB Image

Orthogonal

NDP52 antibody detects NDP52 protein by western blot analysis. Un-treated (-) and treated (+, Thapsigargin treatment for 12hrs and 24hrs) Huh-7 whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody (GTX115378) diluted by 1:2000.
The ACTB was used as internal control (GTX110564, 1:50000) shown at the bottom panel.
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX115378_40302_20160721_WB_shRNA_watermark_18121410_267.jpg

GTX115378 WB Image

KO/KD

Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody (GTX115378) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX115378_40302_20141204_WB_Thapsigargin_2_18121410_627.jpg

GTX115378 WB Image

Orthogonal

NDP52 antibody detects NDP52 protein by western blot analysis. Un-treated (-) and treated (+, Thapsigargin treatment for 12hrs and 24hrs) HepG2 whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody (GTX115378) diluted by 1:2000.
The ACTB was used as internal control (GTX110564, 1:50000) shown at the bottom panel.
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX115378_40302_WB_18121410_249.jpg

GTX115378 WB Image

Sample (30 µg of whole cell lysate)
A: Raji
10% SDS PAGE
GTX115378 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX115378_40302_IFA_18121410_194.jpg

GTX115378 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using NDP52(GTX115378) antibody at 1:200 dilution.

Reference
REVIEW

NDP52 antibody Cat No. GTX115378

Rating ( Average 4 based on 1 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 03-May-2017
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : HeLa cells
Blocking : 5% BSA+10 mM glycine in PBS, 25°C, 1Hr
Fixation : 4% Paraformaldehyde in PBS
Permeabilization /Antigen retrieval : 0.1% Triton X in PBS
Primary Antibody : 1:500, 4°C, overnightHr
SDS
Glycerol.pdf
Thimerosal.pdf