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NDP52 antibody

Anti-NDP52 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115378
Anti-NDP52 antibody used in Western Blot (WB). GTX115378
Anti-NDP52 antibody used in Immunoprecipitation (IP). GTX115378
Anti-NDP52 antibody used in Western Blot (WB). GTX115378
Anti-NDP52 antibody used in IHC (Paraffin sections) (IHC-P). GTX115378
Anti-NDP52 antibody used in Western Blot (WB). GTX115378
Anti-NDP52 antibody used in Western Blot (WB). GTX115378
Anti-NDP52 antibody used in Western Blot (WB). GTX115378
Anti-NDP52 antibody used in Western Blot (WB). GTX115378
Anti-NDP52 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115378
Anti-NDP52 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115378

Cat. No. GTX115378

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

Raji , HepG2 (3 μM Thapsigargin treatment for 12 hr) , HepG2 (3 μM Thapsigargin treatment for 24 hr) , Huh-7 (3 μM Thapsigargin treatment for 12 hr) , Huh-7 (3 μM Thapsigargin treatment for 24 hr)

Predict Reactivity

Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.57 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NDP52. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10620266

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

calcium binding and coiled-coil domain 2 , NDP52

Cellular Localization

Cytoplasm , perinuclear region , Golgi apparatus , cytoskeleton

Background

The protein encoded by this gene is a subunit of nuclear domain 10 (ND10) bodies. ND10 bodies are nuclear domains appearing immunohistochemically as ten dots per nucleus. They are believed to be associated with the nuclear matrix on the basis of their resistance to nuclease digestion and salt extraction. ND10 proteins are removed from the nucleus by herpes simplex virus-1 infection and may have a role in viral life cycles. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-NDP52 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115378

GTX115378 ICC/IF Image

NDP52 antibody detects NDP52 protein at autophagosome  by immunofluorescent analysis.
Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NDP52 stained by NDP52 antibody (GTX115378) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.

Anti-NDP52 antibody used in Western Blot (WB). GTX115378

GTX115378 WB Image

Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody (GTX115378) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NDP52 antibody used in Immunoprecipitation (IP). GTX115378

GTX115378 IP Image

Immunoprecipitation of NDP52 protein from Jurkat whole cell extracts using 5 μg of NDP52 antibody (GTX115378).
Western blot analysis was performed using NDP52 antibody (GTX115378).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-NDP52 antibody used in Western Blot (WB). GTX115378

GTX115378 WB Image

Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody (GTX115378) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NDP52 antibody used in IHC (Paraffin sections) (IHC-P). GTX115378

GTX115378 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H441 xenograft, using NDP52(GTX115378) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NDP52 antibody used in Western Blot (WB). GTX115378

GTX115378 WB Image

NDP52 antibody detects NDP52 protein by western blot analysis. Un-treated (-) and treated (+, Thapsigargin treatment for 12hrs and 24hrs) HepG2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody (GTX115378) diluted by 1:2000.
The ACTB was used as internal control (GTX110564, 1:50000) shown at the bottom panel.
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NDP52 antibody used in Western Blot (WB). GTX115378

GTX115378 WB Image

Sample (30 μg of whole cell lysate)
A: Raji
10% SDS PAGE
GTX115378 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NDP52 antibody used in Western Blot (WB). GTX115378

GTX115378 WB Image

NDP52 antibody detects NDP52 protein by western blot analysis. Un-treated (-) and treated (+, Thapsigargin treatment for 12hrs and 24hrs) Huh-7 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody (GTX115378) diluted by 1:2000.
The ACTB was used as internal control (GTX110564, 1:50000) shown at the bottom panel.
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NDP52 antibody used in Western Blot (WB). GTX115378

GTX115378 WB Image

The data was published in the journal Nat Commun in 2017. PMID: 29187741

Anti-NDP52 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115378

GTX115378 ICC/IF Image

The data was published in the journal Nat Commun in 2017. PMID: 29187741

Anti-NDP52 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115378

GTX115378 ICC/IF Image

The data was published in the journal Dev Cell in 2019.PMID: 31353311

REFERENCE

REVIEW

NDP52 antibody Cat. No. GTX115378

Rating ( Average 4 based on 1 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 03-May-2017
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : HeLa cells
Blocking : 5% BSA+10 mM glycine in PBS, 25°C, 1Hr
Fixation : 4% Paraformaldehyde in PBS
Permeabilization /Antigen retrieval : 0.1% Triton X in PBS
Primary Antibody : 1:500, 4°C, 16Hr
Application Reference
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169