APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.1-0.3μg/ml |
| 2.5μg/ml |
|
Note :
IHC-P
Human hepatocelluar carcinoma shows strong labeling of membranes and weaker staining of cytoplasm. These observations are consistent with those obtained by different antibodies.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX41727-PEP).
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence GNSAGPKSMEVSC, from the C Terminus of the protein sequence according to NP_006087.2.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
N-myc downstream regulated 1 , CAP43 , CMT4D , DRG-1 , DRG1 , GC4 , HMSNL , NDR1 , NMSL , PROXY1 , RIT42 , RTP , TARG1 , TDD5
Cellular Localization
Cytoplasm, cytosol , Nucleus , Cell membrane
Background
This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein involved in stress responses, hormone responses, cell growth, and differentiation. The encoded protein is necessary for p53-mediated caspase activation and apoptosis. Mutations in this gene are a cause of Charcot-Marie-Tooth disease type 4D, and expression of this gene may be a prognostic indicator for several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]
Database
Research Area
DATA IMAGES
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GTX41727 IHC-P Image
IHC-P analysis of human prostate using GTX41727 NDRG1 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 2.5μg/ml
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GTX41727 WB Image
WB analysis of human kidney (A), cerebellum (B) and testis (C) lysate using GTX41727 NDRG1 antibody, C-term.
Dilution : 0.1μg/ml
Loading : 35μg protein in RIPA buffer
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GTX41727 IHC-P Image
IHC-P analysis of human colon using GTX41727 NDRG1 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 2.5μg/ml
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GTX41727 IHC-P Image
IHC-P analysis of human hepatocelluar carcinoma using GTX41727 NDRG1 antibody, C-term.
Dilution : 0.5μg/ml
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REFERENCE
There are currently no references for NDRG1 antibody, C-term (GTX41727). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for NDRG1 antibody, C-term (GTX41727). Be the first to share your experience with this product.
