GeneTex
United States (US)

NDRG1 antibody

GTX100537_39721_WB_19011506_503.jpg
GTX100537_WB_M_19011506_749.jpg
GTX100537_39721_IHC_19011506_816.jpg

Cat No. GTX100537

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

293T , HepG2 , NIH-3T3

Predict Reactivity

Rat, Bovine, Dog, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.67mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NDRG1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

N-Myc Downstream Regulated 1,Cap43,Cmt4D,Drg-1,Drg1,Gc4,Hmsnl,Ndr1,Nmsl,Proxy1,Rit42,Rtp,Targ1,Tdd5,Ndrg1

Cellular Localization

Cytoplasm , Nucleus , Cell membrane

Background

This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein involved in stress responses, hormone responses, cell growth, and differentiation. It is necessary for p53-mediated caspase activation and apoptosis. Mutation in this gene has been reported to be causative for hereditary motor and sensory neuropathy-Lom. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100537_39721_WB_19011506_503.jpg

GTX100537 WB Image

NDRG1 antibody detects NDRG1 protein by Western blot analysis.
A. 30 µg 293T whole cell lysate/extract
B. 30 µg HepG2 whole cell lysate/extract
10 % SDS-PAGE
NDRG1 antibody (GTX100537) dilution: 1:1000

GTX100537_WB_M_19011506_749.jpg

GTX100537 WB Image

Sample (30 ug of whole cell lysate)

A:NIH-3T3
10% SDS PAGE

GTX100537 diluted at 1:1000

GTX100537_39721_IHC_19011506_816.jpg

GTX100537 IHC-P Image

NDRG1 antibody detects NDRG1 protein at membrane on human liver carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded liver carcinoma.
NDRG1 antibody (GTX100537) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf