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NDRG1 antibody

Anti-NDRG1 antibody used in Western Blot (WB). GTX100537
Anti-NDRG1 antibody used in Western Blot (WB). GTX100537
Anti-NDRG1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100537
Anti-NDRG1 antibody used in Western Blot (WB). GTX100537

Cat No. GTX100537

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

293T , HepG2 , NIH-3T3

Predict Reactivity

Bovine, Dog, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.67 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NDRG1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

N-myc downstream regulated 1 , CAP43 , CMT4D , DRG-1 , DRG1 , GC4 , HMSNL , NDR1 , NMSL , PROXY1 , RIT42 , RTP , TARG1 , TDD5

Cellular Localization

Cytoplasm , Nucleus , Cell membrane

Background

This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein involved in stress responses, hormone responses, cell growth, and differentiation. It is necessary for p53-mediated caspase activation and apoptosis. Mutation in this gene has been reported to be causative for hereditary motor and sensory neuropathy-Lom. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-NDRG1 antibody used in Western Blot (WB). GTX100537

GTX100537 WB Image

NDRG1 antibody detects NDRG1 protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg HepG2 whole cell lysate/extract
10 % SDS-PAGE
NDRG1 antibody (GTX100537) dilution: 1:1000

Anti-NDRG1 antibody used in Western Blot (WB). GTX100537

GTX100537 WB Image

Sample (30 ug of whole cell lysate)

A:NIH-3T3
10% SDS PAGE

GTX100537 diluted at 1:1000

Anti-NDRG1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100537

GTX100537 IHC-P Image

NDRG1 antibody detects NDRG1 protein at membrane on human liver carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded liver carcinoma.
NDRG1 antibody (GTX100537) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NDRG1 antibody used in Western Blot (WB). GTX100537

GTX100537 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with NDRG1 antibody (GTX100537) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169